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Direct electrochemistry of horseradish peroxidase immobilized on a monolayer modified nanowire array electrode
Vertically aligned nanowire array electrodes (NAEs) were prepared by electrodeposition of gold into an anodic aluminium oxide membrane (AAM), providing an ordered three-dimensional (3D) matrix for immobilization of redox proteins. Third-generation H 2O 2 biosensors were prepared by covalent immobili...
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| Published in: | Biosensors & bioelectronics 2010-02, Vol.25 (6), p.1313-1318 |
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| Main Authors: | , , , , |
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| cites | cdi_FETCH-LOGICAL-c592t-e16be4c873acd58ce5acd48f4ed58fa4d2211166453d0d5a9bd0d332ea0580d83 |
| container_end_page | 1318 |
| container_issue | 6 |
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| container_title | Biosensors & bioelectronics |
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| creator | Xu, Ju Shang, Fengjun Luong, John H.T. Razeeb, Kafil M. Glennon, Jeremy D. |
| description | Vertically aligned nanowire array electrodes (NAEs) were prepared by electrodeposition of gold into an anodic aluminium oxide membrane (AAM), providing an ordered three-dimensional (3D) matrix for immobilization of redox proteins. Third-generation H
2O
2 biosensors were prepared by covalent immobilization of horseradish peroxidase (HRP) on the self-assembled monolayer modified NAEs. Direct electron transfer and electrocatalytic performances of the HRP/NAEs with different nanowire lengths (deposition time of 2, 4 and 5
h) were investigated. Results showed that with longer nanowires, better performances were achieved. The HRP/NAE
5
h
(5
h deposition time) exhibited remarkable sensitivity (45.86
μA
mM
−1
cm
−2) towards H
2O
2 with a detection limit of 0.42
μM (
S/
N
=
3), linearity up to 15
mM and a response time of 4
s. The ordered 3D gold nanowire array with high conductivity, excellent electron transfer capability and good biocompatibility proved promising for fabricating sensitive, selective, stable and mediator-free enzymatic biosensors. |
| doi_str_mv | 10.1016/j.bios.2009.10.018 |
| format | article |
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2O
2 biosensors were prepared by covalent immobilization of horseradish peroxidase (HRP) on the self-assembled monolayer modified NAEs. Direct electron transfer and electrocatalytic performances of the HRP/NAEs with different nanowire lengths (deposition time of 2, 4 and 5
h) were investigated. Results showed that with longer nanowires, better performances were achieved. The HRP/NAE
5
h
(5
h deposition time) exhibited remarkable sensitivity (45.86
μA
mM
−1
cm
−2) towards H
2O
2 with a detection limit of 0.42
μM (
S/
N
=
3), linearity up to 15
mM and a response time of 4
s. The ordered 3D gold nanowire array with high conductivity, excellent electron transfer capability and good biocompatibility proved promising for fabricating sensitive, selective, stable and mediator-free enzymatic biosensors.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2009.10.018</identifier><identifier>PMID: 19914056</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Biological and medical sciences ; Biosensing Techniques - instrumentation ; Biotechnology ; Direct electrochemistry ; Electrochemistry - instrumentation ; Enzymes, Immobilized - chemistry ; Equipment Design ; Equipment Failure Analysis ; Fundamental and applied biological sciences. Psychology ; Horseradish peroxidase ; Horseradish Peroxidase - chemistry ; Hydrogen Peroxide - analysis ; Hydrogen Peroxide - chemistry ; Microelectrodes ; Nanotechnology - instrumentation ; Nanotubes - chemistry ; Nanotubes - ultrastructure ; Nanowire array electrode ; Reproducibility of Results ; Self-assembled monolayer ; Sensitivity and Specificity</subject><ispartof>Biosensors & bioelectronics, 2010-02, Vol.25 (6), p.1313-1318</ispartof><rights>2009 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright 2009 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c592t-e16be4c873acd58ce5acd48f4ed58fa4d2211166453d0d5a9bd0d332ea0580d83</citedby><cites>FETCH-LOGICAL-c592t-e16be4c873acd58ce5acd48f4ed58fa4d2211166453d0d5a9bd0d332ea0580d83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22536818$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19914056$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Ju</creatorcontrib><creatorcontrib>Shang, Fengjun</creatorcontrib><creatorcontrib>Luong, John H.T.</creatorcontrib><creatorcontrib>Razeeb, Kafil M.</creatorcontrib><creatorcontrib>Glennon, Jeremy D.</creatorcontrib><title>Direct electrochemistry of horseradish peroxidase immobilized on a monolayer modified nanowire array electrode</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>Vertically aligned nanowire array electrodes (NAEs) were prepared by electrodeposition of gold into an anodic aluminium oxide membrane (AAM), providing an ordered three-dimensional (3D) matrix for immobilization of redox proteins. Third-generation H
2O
2 biosensors were prepared by covalent immobilization of horseradish peroxidase (HRP) on the self-assembled monolayer modified NAEs. Direct electron transfer and electrocatalytic performances of the HRP/NAEs with different nanowire lengths (deposition time of 2, 4 and 5
h) were investigated. Results showed that with longer nanowires, better performances were achieved. The HRP/NAE
5
h
(5
h deposition time) exhibited remarkable sensitivity (45.86
μA
mM
−1
cm
−2) towards H
2O
2 with a detection limit of 0.42
μM (
S/
N
=
3), linearity up to 15
mM and a response time of 4
s. The ordered 3D gold nanowire array with high conductivity, excellent electron transfer capability and good biocompatibility proved promising for fabricating sensitive, selective, stable and mediator-free enzymatic biosensors.</description><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biotechnology</subject><subject>Direct electrochemistry</subject><subject>Electrochemistry - instrumentation</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Horseradish peroxidase</subject><subject>Horseradish Peroxidase - chemistry</subject><subject>Hydrogen Peroxide - analysis</subject><subject>Hydrogen Peroxide - chemistry</subject><subject>Microelectrodes</subject><subject>Nanotechnology - instrumentation</subject><subject>Nanotubes - chemistry</subject><subject>Nanotubes - ultrastructure</subject><subject>Nanowire array electrode</subject><subject>Reproducibility of Results</subject><subject>Self-assembled monolayer</subject><subject>Sensitivity and Specificity</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqFkU1PGzEQhq2qqKS0f6AH5EvV0wZ_767UC0o_qITEhZ6tWXtWcbS7Tu2kkP56HBLoDS4e-9Uzr2b8EvKJszln3Fys5l2IeS4Ya4swZ7x5Q2a8qWWlhNRvyYy12lTaGHlK3ue8YozVvGXvyClvW66YNjMyfQsJ3YbiUM4U3RLHkDdpR2NPlzFlTOBDXtI1pngfPGSkYRxjF4bwDz2NEwU6xikOsMNUbj70oegTTPGuOFNICXZP7h4_kJMehowfj_WM_P7x_XZxVV3f_Py1uLyunG7FpkJuOlSurALO68ahLlU1vcLy6kF5ITjnxigtPfMa2q4UKQUC0w3zjTwjXw6-6xT_bDFvbFnL4TDAhHGbbVPXulZCv07WUrZMGiULKQ6kSzHnhL1dpzBC2lnO7D4Qu7L7QOw-kL1WAilN50f7bTei_99yTKAAn48AZAdDn2ByIT9zQmhpmkejrwcOy7f9DZhsdgEnh_4xQOtjeGmOB5Pwq_M</recordid><startdate>20100215</startdate><enddate>20100215</enddate><creator>Xu, Ju</creator><creator>Shang, Fengjun</creator><creator>Luong, John H.T.</creator><creator>Razeeb, Kafil M.</creator><creator>Glennon, Jeremy D.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20100215</creationdate><title>Direct electrochemistry of horseradish peroxidase immobilized on a monolayer modified nanowire array electrode</title><author>Xu, Ju ; Shang, Fengjun ; Luong, John H.T. ; Razeeb, Kafil M. ; Glennon, Jeremy D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c592t-e16be4c873acd58ce5acd48f4ed58fa4d2211166453d0d5a9bd0d332ea0580d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biotechnology</topic><topic>Direct electrochemistry</topic><topic>Electrochemistry - instrumentation</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Horseradish peroxidase</topic><topic>Horseradish Peroxidase - chemistry</topic><topic>Hydrogen Peroxide - analysis</topic><topic>Hydrogen Peroxide - chemistry</topic><topic>Microelectrodes</topic><topic>Nanotechnology - instrumentation</topic><topic>Nanotubes - chemistry</topic><topic>Nanotubes - ultrastructure</topic><topic>Nanowire array electrode</topic><topic>Reproducibility of Results</topic><topic>Self-assembled monolayer</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Ju</creatorcontrib><creatorcontrib>Shang, Fengjun</creatorcontrib><creatorcontrib>Luong, John H.T.</creatorcontrib><creatorcontrib>Razeeb, Kafil M.</creatorcontrib><creatorcontrib>Glennon, Jeremy D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Ju</au><au>Shang, Fengjun</au><au>Luong, John H.T.</au><au>Razeeb, Kafil M.</au><au>Glennon, Jeremy D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct electrochemistry of horseradish peroxidase immobilized on a monolayer modified nanowire array electrode</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2010-02-15</date><risdate>2010</risdate><volume>25</volume><issue>6</issue><spage>1313</spage><epage>1318</epage><pages>1313-1318</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>Vertically aligned nanowire array electrodes (NAEs) were prepared by electrodeposition of gold into an anodic aluminium oxide membrane (AAM), providing an ordered three-dimensional (3D) matrix for immobilization of redox proteins. Third-generation H
2O
2 biosensors were prepared by covalent immobilization of horseradish peroxidase (HRP) on the self-assembled monolayer modified NAEs. Direct electron transfer and electrocatalytic performances of the HRP/NAEs with different nanowire lengths (deposition time of 2, 4 and 5
h) were investigated. Results showed that with longer nanowires, better performances were achieved. The HRP/NAE
5
h
(5
h deposition time) exhibited remarkable sensitivity (45.86
μA
mM
−1
cm
−2) towards H
2O
2 with a detection limit of 0.42
μM (
S/
N
=
3), linearity up to 15
mM and a response time of 4
s. The ordered 3D gold nanowire array with high conductivity, excellent electron transfer capability and good biocompatibility proved promising for fabricating sensitive, selective, stable and mediator-free enzymatic biosensors.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>19914056</pmid><doi>10.1016/j.bios.2009.10.018</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0956-5663 |
| ispartof | Biosensors & bioelectronics, 2010-02, Vol.25 (6), p.1313-1318 |
| issn | 0956-5663 1873-4235 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_877574258 |
| source | ScienceDirect Freedom Collection |
| subjects | Biological and medical sciences Biosensing Techniques - instrumentation Biotechnology Direct electrochemistry Electrochemistry - instrumentation Enzymes, Immobilized - chemistry Equipment Design Equipment Failure Analysis Fundamental and applied biological sciences. Psychology Horseradish peroxidase Horseradish Peroxidase - chemistry Hydrogen Peroxide - analysis Hydrogen Peroxide - chemistry Microelectrodes Nanotechnology - instrumentation Nanotubes - chemistry Nanotubes - ultrastructure Nanowire array electrode Reproducibility of Results Self-assembled monolayer Sensitivity and Specificity |
| title | Direct electrochemistry of horseradish peroxidase immobilized on a monolayer modified nanowire array electrode |
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