Amorphous calcium phosphate/poly( d, l-lactic acid) composite nanofibers: Electrospinning preparation and biomineralization

• Published in: Journal of colloid and interface science 2011-07, Vol.359 (2), p.371-379
• Main Authors: Ma, Zhao, Chen, Feng, Zhu, Ying-Jie, Cui, Ting, Liu, Xuan-Yong
• Format: Article
• Language: English
• Subjects: Biocompatibility; Biocompatible Materials - chemistry; Biocompatible Materials - metabolism; Biomedical materials; Biomineralization; Calcification, Physiologic; Calcium phosphate; calcium phosphates; Calcium Phosphates - chemistry; Calcium Phosphates - metabolism; Cell Line; Cell Survival; Chemistry; Colloidal state and disperse state; cytotoxicity; Electrospinning; Equipment Design; Exact sciences and technology; Finite element method; General and physical chemistry; Humans; in vitro studies; Lactic Acid - chemistry; Lactic Acid - metabolism; Nanocomposite; Nanofiber; Nanofibers; Nanofibers - chemistry; Nanofibers - ultrastructure; nanoparticles; Nanostructure; Nanotechnology - instrumentation; Osteoblasts - cytology; Osteoblasts - metabolism; Physical and chemical studies. Granulometry. Electrokinetic phenomena; Poly( d, l-lactic acid); Polyesters; polylactic acid; Polymers - chemistry; Polymers - metabolism
• ISSN: 0021-9797; 1095-7103
• DOI: 10.1016/j.jcis.2011.04.023

The composite nanofibers consisting of amorphous calcium phosphate (ACP) nanoparticles and poly( d, l-lactic acid) (PDLLA) have been prepared by electrospinning, which exhibit a fast mineralization behavior in the simulated body fluid, and a good biocompatibility and bioactivity. [Display omitted] ► An electrospinning preparation of amorphous calcium phosphate (ACP)/poly( d, l-lactic acid) (PDLLA) composite nanofibers is presented. ► Different architectures including the nanofibrous mesh and tube consisting of ACP/PDLLA composite nanofibers were obtained. ► ACP/PDLLA composite nanofibers showed fast mineralization in simulated body fluid, good biocompatibility and bioactivity. ► ACP/PDLLA composite nanofibers have the potential for applications in tissue engineering and other biomedical fields. Amorphous calcium phosphate (ACP) has been recognized as an attractive biomaterial due to its bioactivity and biocompatibility. Electrospinning is a simple and low-cost way to fabricate polymer fibers. In this study, ACP nanoparticles with diameters ranging from 20 to 80 nm were synthesized using a simple precipitation method. ACP nanoparticles were hybridized with poly( d, l-lactic acid) (PDLLA) to form ACP/PDLLA composite nanofibers by electrospinning, and different architectures including the nanofibrous mesh and tube consisting of ACP/PDLLA composite nanofibers were obtained and characterized. The biomineralization and cytocompatibility of as-prepared ACP/PDLLA composite nanofibers were evaluated in vitro. Osteoblast-like MG63 cells were seeded on the ACP/PDLLA composite nanofiber meshes to perform the cytocompatibility evaluation. The ACP/PDLLA composite nanofibers exhibited a fast mineralization behavior in the simulated body fluid. The attachment of MG63 cells and cytotoxicity of ACP/PDLLA composite nanofibers were also evaluated, and the experiments indicated good biocompatibility and bioactivity of ACP/PDLLA composite nanofibers.