Inactivation of Staphylococcus aureus in Water by a Cold, He/O2 Atmospheric Pressure Plasma Microjet

A direct‐current, atmospheric pressure, cold plasma microjet (PMJ) sustained in a quasi‐steady gas cavity in liquid was used to inactivate Staphylococcus aureus suspended in distilled water. While helium gas (with 2% O2 as additive) was used as working gas, an effective inactivation (>99%) was ac...

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Bibliographic Details
Published in:Plasma processes and polymers 2011-05, Vol.8 (5), p.424-431
Main Authors: Bai, Na, Sun, Peng, Zhou, Haixia, Wu, Haiyan, Wang, Ruixue, Liu, Fuxiang, Zhu, Weidong, Lopez, Jose L., Zhang, Jue, Fang, Jing
Format: Article
Language:English
Subjects:
Applied microbiology
Applied sciences
atmospheric pressure
Biological and medical sciences
Drinking water and swimming-pool water. Desalination
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
inactivation
Microbiology
oxygen species
plasma microjet
Pollution
Staphylococcus aureus
Sterilization methods
Water treatment and pollution
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Summary:A direct‐current, atmospheric pressure, cold plasma microjet (PMJ) sustained in a quasi‐steady gas cavity in liquid was used to inactivate Staphylococcus aureus suspended in distilled water. While helium gas (with 2% O2 as additive) was used as working gas, an effective inactivation (>99%) was achieved in 6 min. The inactivation of bacteria was further verified by surface morphology examination and LIVE/DEAD Baclight bacterial viability test (fluorescence microscopy). The overall pH and temperature of the liquid were monitored during the plasma treatment and were found to be below the critical values for the survival of S. aureus. Hydroxyl radical (•OH) was detected via electron spin resonance (ESR) spectroscopy, and alongside other intermediate reactive species, is attributed to the effective inactivation of S. aureus. End‐on optical emission spectroscopy show strong atomic oxygen emission both in air and in water. A DC, microhollow cathode discharge based plasma microjet with He and 2% oxygen as working gas was used to treat Staphylococcus aureus suspended in distilled water. Effective inactivation (>99%) of bacteria was achieved in ∼6min, which were confirmed by SEM and LIVE/DEAD Baclight bacterial viability test. OES and ESR spectroscopy show strong oxygen emission and hydroxyl generation in water.
ISSN:1612-8850
1612-8869
1612-8869
DOI:10.1002/ppap.201000078