Mitotic catastrophe induced by overexpression of budding yeast Rad2p

Mitotic catastrophe provokes endopolyploidy, giant cell formation and, eventually, delayed cell death. Mitotic catastrophe is induced by defective cell cycle checkpoints and by some anticancer drugs, ionizing radiation and microtubule-destabilizing agents. RAD2 is a yeast homologue of XPG, which is...

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Published in:Yeast (Chichester, England) England), 2010-07, Vol.27 (7), p.399-411
Main Authors: Kang, Mi-Sun, Yu, Sung-Lim, Lim, Hyun-Sook, Choi, Bohwa, Park, Chang-Shin, Kang, Ju-Hee, Lee, Sung-Keun
Format: Article
Language:English
Subjects:
Caspases - genetics
Cell Cycle Proteins - genetics
DNA-Binding Proteins - biosynthesis
DNA-Binding Proteins - genetics
Endodeoxyribonucleases - biosynthesis
Endodeoxyribonucleases - genetics
Gene Deletion
Gene Expression
Mitosis
mitotic catastrophe
nucleotide excision repair
RAD2
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - physiology
Saccharomyces cerevisiae Proteins - biosynthesis
Saccharomyces cerevisiae Proteins - genetics
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Summary:Mitotic catastrophe provokes endopolyploidy, giant cell formation and, eventually, delayed cell death. Mitotic catastrophe is induced by defective cell cycle checkpoints and by some anticancer drugs, ionizing radiation and microtubule-destabilizing agents. RAD2 is a yeast homologue of XPG, which is a human endonuclease involved in nucleotide excision repair. Here we show that Rad2p overexpression alone, in the absence of extrinsic DNA damage, causes cell growth arrest and mitotic catastrophe. Interestingly, Rad2p-induced cell growth arrest is not caused by the catalytic activity of Rad2p but rather by its C-terminal region. Cells growth-arrested by Rad2p induction do not show apoptotic phenotypes and deletion of YCA1, a yeast caspase homologue, does not affect cell growth arrest by Rad2p induction. However, Rad2p-induced cell growth arrest is released by rad9 deletion but is not affected by downstream DNA damage checkpoint genes. These observations suggest that RAD2 has a function in coordinating cell cycle regulation and damaged DNA repair. Copyright © 2010 John Wiley & Sons, Ltd.
ISSN:0749-503X
1097-0061
1097-0061
DOI:10.1002/yea.1764