Association of secondhand smoke exposures with DNA methylation in bladder carcinomas

• Published in: Cancer causes & control 2011-08, Vol.22 (8), p.1205-1213
• Main Authors: Wilhelm-Benartzi, Charlotte S., Christensen, Brock C., Koestler, Devin, Houseman, E. Andres, Schned, Alan R., Karagas, Margaret R., Kelsey, Karl T., Marsit, Carmen J.
• Format: Article
• Language: English
• Subjects: Adulthood; Biomedical and Life Sciences; Biomedicine; Bladder cancer; BRIEF REPORT; Cancer; Cancer Research; Carcinogenesis; Carcinogens; Carcinoma; Childhood; Children; Cigarette smoking; Cigarettes; CpG islands; DNA; DNA Methylation; Environmental Exposure; Epidemiology; Epigenetics; Female; Genetic loci; Hematology; Humans; Immunomodulation; Male; Methylation; Middle Aged; Occupational exposure; Oncology; Passive smoking; Public Health; Risk factors; Secondhand smoke; Signal transduction; Smoke; Smoking; Tobacco smoke; Tobacco Smoke Pollution - adverse effects; Tobacco smoking; Tumors; Urinary bladder; Urinary Bladder Neoplasms - etiology; Urinary Bladder Neoplasms - genetics
• ISSN: 0957-5243; 1573-7225
• DOI: 10.1007/s10552-011-9788-6

Background: The association between secondhand smoke (SHS) exposure and bladder cancer is inconclusive. Epigenetic alterations in bladder tumors have been linked to primary cigarette smoking and could add to the biological plausibility of an association between SHS exposure and bladder cancer. Hypothesis: SHS exposure is associated with DNA methylation in bladder tumors. Methods: Using an array-based approach, we profiled DNA methylation from never smoking cases of incident bladder cancer. Analyses examined associations between individual loci's methylation with SHS variables (exposure in adulthood, childhood, occupationally, and total exposure). A canonical pathway analysis was used to find pathways significantly associated with each SHS exposure type. Results: There is a trend toward increased methylation of numerous CpG loci with increasing exposure to adulthood, occupational, and total SHS. Discrete associations between methylation extent of several CpG loci and SHS exposures demonstrated significantly increased methylation of these loci across all types of SHS exposure. CpGs with SHSrelated methylation alterations were associated with genes in pathways involved in carcinogenesis, immune modulation, and immune signaling. Interpretation: Exposures to SHS in adulthood, childhood, occupationally, and in total are each significantly associated with changes in DNA methylation of several CpG loci in bladder tumors, adding biological plausibility to SHS as a risk factor for bladder cancer.