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Microglial cystatin F expression is a sensitive indicator for ongoing demyelination with concurrent remyelination
Demyelination coincides with numerous changes of gene expression in the central nervous system (CNS). Cystatin F, which is a papain‐like lysosomal cysteine proteinase inhibitor that is normally expressed by immune cells and not in the brain, is massively induced in the CNS during acute demyelination...
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Published in: | Journal of neuroscience research 2011-05, Vol.89 (5), p.639-649 |
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Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Demyelination coincides with numerous changes of gene expression in the central nervous system (CNS). Cystatin F, which is a papain‐like lysosomal cysteine proteinase inhibitor that is normally expressed by immune cells and not in the brain, is massively induced in the CNS during acute demyelination. We found that microglia, which are monocyte/macrophage‐lineage cells in the CNS, express cystatin F only during demyelination. By using several demyelinating animal models and the spinal cord tissues from multiple sclerosis (MS) patients, we examined spatiotemporal expression pattern of cystatin F by in situ hybridization and immunohistochemistry. We found that the timing of cystatin F induction matches with ongoing demyelination, and the places with cystatin F expression overlapped with the remyelinating area. Most interestingly, cystatin F induction ceased in chronic demyelination, in which remyelinating ability was lost. These findings demonstrate that the expression of cystatin F indicates the occurrence of ongoing demyelination/remyelination and the absence of cystatin F expression indicates the cessation of remyelination in the demyelinating area. © 2011 Wiley‐Liss, Inc. |
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ISSN: | 0360-4012 1097-4547 1097-4547 |
DOI: | 10.1002/jnr.22567 |