use of meat juice or blood serum for the diagnosis of Salmonella infection in pigs and its possible implications on Salmonella control programs

Serology is the method of choice for country-scale Salmonella control programs in pigs and can be carried out both on blood serum or meat juice. However, the diagnostic performance of enzyme-linked immunosorbent assays (ELISAs) on these sample matrices has not been sufficiently compared. The agreeme...

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Bibliographic Details
Published in:Journal of veterinary diagnostic investigation 2011-05, Vol.23 (3), p.528-531
Main Authors: Vico, Juan P, Mainar-Jaime, Raul C
Format: Article
Language:English
Subjects:
absorbance
Animals
blood serum
commercial farms
correlation
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - veterinary
Female
Meat - microbiology
meat juices
Salmonella
Salmonella Infections, Animal - blood
Salmonella Infections, Animal - diagnosis
Salmonella Infections, Animal - prevention & control
Sensitivity and Specificity
sows
Swine - microbiology
Swine Diseases - blood
Swine Diseases - diagnosis
Swine Diseases - microbiology
Swine Diseases - prevention & control
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Summary:Serology is the method of choice for country-scale Salmonella control programs in pigs and can be carried out both on blood serum or meat juice. However, the diagnostic performance of enzyme-linked immunosorbent assays (ELISAs) on these sample matrices has not been sufficiently compared. The agreement between the serum ELISA and meat juice ELISA on samples taken from commercial farms was assessed in 2 pig populations (adult sows and finishers). Results of optical density percentage (OD%) from the serum ELISA were consistently higher than those from the meat juice ELISA (38.5 vs. 27.9; P < 0.001), and the mean difference between them was significantly different from zero (P < 0.0001). The overall correlation coefficient between serum ELISA and meat juice ELISA results was low (r = 0.53). These results indicated an important disagreement between ELISA performed on serum and meat juice matrices and suggested that before implementing a control program to reduce the prevalence of Salmonella in swine the choice of matrix on which to perform the ELISA should be carefully considered.
ISSN:1040-6387
1943-4936
DOI:10.1177/1040638711403432