Impedance-Based Surveillance of Transient Permeability Changes in Coronary Endothelial Monolayers after Exposure to Ionizing Radiation

The relative radiation sensitivities of the various compartments of the heart are poorly characterized. Cardiac fibrosis is a common side effect of radiotherapy, suggesting that endothelial barrier function is an important factor in radiation-induced pathology. We employed Electric Cell Substrate Im...

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Bibliographic Details
Published in:Radiation research 2011-10, Vol.176 (4), p.415-424
Main Authors: Young, Erik F., Smilenov, Lubomir B.
Format: Article
Language:English
Subjects:
Cell Movement - radiation effects
Coronary Vessels - cytology
Cytoskeleton - radiation effects
Electric Impedance
Electrodes
Endothelial cells
Endothelium
Endothelium, Vascular - cytology
Endothelium, Vascular - metabolism
Endothelium, Vascular - radiation effects
Humans
Ionizing radiation
Irradiation
Kinetics
Models, Biological
Molecular Imaging
Permeability - radiation effects
Radiation damage
Radiation dosage
Radiotherapy
REGULAR ARTICLES
Space life sciences
Statistical significance
Time Factors
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Summary:The relative radiation sensitivities of the various compartments of the heart are poorly characterized. Cardiac fibrosis is a common side effect of radiotherapy, suggesting that endothelial barrier function is an important factor in radiation-induced pathology. We employed Electric Cell Substrate Impedance Sensing (ECIS) to assess cytoskeletal rearrangement, permeability changes and endothelial barrier function changes in response to radiation in studies of human coronary arterial endothelial cells (HCAECs). A 5-Gy dose of γ radiation resulted in a significant sixfold transient decrease in transmonolayer resistance 3 h postirradiation (P = 0.001). This decrease in resistance coincided with changes in fluorescent tracer flux (P = 0.05) and display of an actin bundling phenotype. After irradiation, decreases in wound healing (P = 0.03) and micromotion within the monolayer (P = 0.02) were also observed. Time-lapse video studies confirmed that the monolayer is dynamic and showed that cells are extruded from the monolayer at a higher frequency after irradiation. These findings suggest that perturbed endothelial barrier function in the heart can occur at lower doses of γ radiation than previously reported.
ISSN:0033-7587
1938-5404
DOI:10.1667/RR2665.1