Human Papillomavirus genotype testing combined with cytology as a ‘test of cure’ post treatment: The importance of a persistent viral infection

• Published in: Journal of clinical virology 2011-10, Vol.52 (2), p.88-92
• Main Authors: Jones, Joanne, Saleem, Azka, Rai, Nima, Shylasree, T.S, Ashman, Suzanne, Gregory, Kate, Powell, Ned, Tristram, Amanda, Fiander, Alison, Hibbitts, Samantha
• Format: Article
• Language: English
• Subjects: Adult; Allergy and Immunology; Biological and medical sciences; Cervical Intraepithelial Neoplasia - diagnosis; Cervical Intraepithelial Neoplasia - surgery; Cervical Intraepithelial Neoplasia - virology; Cervical screening; Cytological Techniques; Female; Fundamental and applied biological sciences. Psychology; Genotyping Techniques; HPV genotyping; Human papillomavirus; Human papillomavirus 16; Human viral diseases; Humans; Infectious Disease; Infectious diseases; Medical sciences; Microbiology; Middle Aged; Miscellaneous; Nuclear polyhedrosis virus; PapilloCheck; Papillomaviridae - genetics; Papillomavirus Infections - diagnosis; Papillomavirus Infections - surgery; Papillomavirus Infections - virology; Recurrence; Sensitivity and Specificity; Test of cure; Uterine Cervical Neoplasms - diagnosis; Uterine Cervical Neoplasms - surgery; Uterine Cervical Neoplasms - virology; Viral diseases; Virology; Young Adult
• ISSN: 1386-6532; 1873-5967
• DOI: 10.1016/j.jcv.2011.06.021

Abstract Background Human Papillomavirus (HPV) testing has been evaluated as a test of cure in patients following treatment of high-grade cervical intraepithelial neoplasia (CIN2+). Studies show that women who are HPV and cytology negative post treatment can be safely returned to routine recall. The management strategy for HPV positive women requires confirmation. Objective To evaluate the clinical utility of the PapilloCheck® genotyping assay for predicting disease recurrence in a test of cure setting. Study design Ninety-eight women (19–52 years) treated for CIN2+ by large loop excision of the transformation zone (LLETZ) were evaluated with samples taken before and 6 months after treatment for HPV testing. Cytology and histology were available from recruitment until 24 months post treatment. Results Recurrent disease was evident in 4% of patients with 2 cases low-grade and 2 cases of high-grade disease. In women with no disease recurrence, 40% (95% CI 30.42–51.05%) were high risk (HR) HPV negative post LLETZ. Both cases with high-grade disease had persistent HPV16 infection. Genotyping before and after treatment revealed 83% (95% CI 75.74–88.78%) of total viral infections were cleared and 17% (95% CI 11.22–24.26) viral infections persisted. Post treatment, combined cytology and HPV test results predicted CIN2+ with 100% sensitivity, 91.7% specificity, 100% NPV and 20% PPV and measuring viral persistence marginally increased specificity and PPV. Conclusion Post treatment, cytology combined with a single HR HPV test has high sensitivity and specificity for predicting disease recurrence. HPV genotyping before and after LLETZ identifies persistent viral infections and could help refine patient management.