T cell deficiency does not reduce lesions in mice produced by intracerebral injection of NMO-IgG and complement

Abstract We reported recently that intracerebral administration of NMO-IgG with human complement produces neuromyelitis optica (NMO) lesions in mice. We examined the role of T cells in the formation of NMO lesions by comparing brain histopathology in wildtype and nude mice. Brains were co-injected w...

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Bibliographic Details
Published in:Journal of neuroimmunology 2011-06, Vol.235 (1), p.27-32
Main Authors: Saadoun, Samira, Waters, Patrick, MacDonald, Claire, Bridges, Leslie R, Bell, B. Anthony, Vincent, Angela, Verkman, A.S, Papadopoulos, Marios C
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Aquaporin-4
Brain - immunology
Brain - pathology
Complement System Proteins - administration & dosage
Devic's syndrome
Humans
Immunoglobulin G - administration & dosage
Immunologic Deficiency Syndromes - immunology
Immunologic Deficiency Syndromes - pathology
Injections, Intraventricular
Lymphocytes
Mice
Mice, Knockout
Mice, Nude
Neurology
Neuromyelitis Optica - immunology
Neuromyelitis Optica - pathology
NMO
T cells
T-Lymphocytes - immunology
T-Lymphocytes - pathology
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Summary:Abstract We reported recently that intracerebral administration of NMO-IgG with human complement produces neuromyelitis optica (NMO) lesions in mice. We examined the role of T cells in the formation of NMO lesions by comparing brain histopathology in wildtype and nude mice. Brains were co-injected with IgG from NMO patients and human complement. At 24 h and 5 days, wildtype vs. nude mouse brains had comparable inflammation (CD45 immunoreactivity), loss of myelin (Luxol Fast Blue staining) and loss of AQP4 immunoreactivity. We conclude that T cells are not required for the formation of NMO lesions in this mouse model.
ISSN:0165-5728
1872-8421
DOI:10.1016/j.jneuroim.2011.03.007