Single nucleotide polymorphisms in collagenous lectins and other innate immune genes in pigs with common infectious diseases

• Published in: Veterinary immunology and immunopathology 2011-07, Vol.142 (1), p.1-13
• Main Authors: Keirstead, N.D., Hayes, M.A., Vandervoort, G.E., Brooks, A.S., Squires, E.J., Lillie, B.N.
• Format: Article
• Language: English
• Subjects: Animals; Collagenous lectin (collectin); Collectins - genetics; Communicable Diseases - genetics; Communicable Diseases - immunology; Communicable Diseases - microbiology; Communicable Diseases - veterinary; Communicable Diseases - virology; Ficolin; Ficolins; Galectin 4 - genetics; Genotype; Immunity, Innate - genetics; Immunity, Innate - immunology; Lectins - genetics; Mannan-binding lectin (MBL); Mannose-Binding Protein-Associated Serine Proteases - genetics; Pig (porcine); Polymorphism, Single Nucleotide - genetics; Polymorphism, Single Nucleotide - immunology; Single nucleotide polymorphism (SNP); Surfactant protein-A (SP-A); Swine - genetics; Swine - immunology; Swine - microbiology; Swine Diseases - genetics; Swine Diseases - immunology; Swine Diseases - microbiology
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/j.vetimm.2011.02.011

Innate immune recognition of pathogens involves various surface receptors and soluble proteins that precede agglutination, complement activation, phagocytosis, and the adaptive immune response. Mannan-binding lectins (MBLs), ficolins (FCNs) and surfactant protein A (SP-A) are soluble collagenous lectins that bind surface structures of various bacteria, viruses and fungi. Some single nucleotide polymorphisms (SNPs) in collagenous lectin genes of humans and other species, including pigs, have been implicated in variation in susceptibility to infectious and inflammatory diseases. In this study we determined the frequencies of 13 SNP alleles of MBL-A, MBL-C, ficolin-α, ficolin-β, and SP-A in 1324 healthy pigs and 461 pigs diagnosed with common infectious diseases at necropsy. For comparison, we also analyzed 12 other SNP alleles in several other innate immune genes, including galectins and TLRs. Several SNPs within genes encoding porcine MBL-A, MBL-C and SP-A were more frequent in pigs diagnosed at necropsy with various diseases or pathogens. These findings suggest that several collagenous lectin SNPs are associated with disease susceptibility and therefore might be genetic markers of impaired innate immune function.