Dopamine up-regulates Th17 phenotype from individuals with generalized anxiety disorder

Abstract Our objective was to evaluate the effect of stress-related dose of dopamine (DA) on the in vitro proliferation and cytokine production in polyclonally-activated T cells from healthy individuals or individuals with generalized anxiety disorder (GAD). Our results demonstrated that cell cultur...

Full description

Saved in:
Bibliographic Details
Published in:Journal of neuroimmunology 2011-09, Vol.238 (1), p.58-66
Main Authors: Ferreira, Thais B, Kasahara, Taissa M, Barros, Priscila O, Vieira, Morgana M.M, Bittencourt, Vera Carolina B, Hygino, Joana, Andrade, Regis M, Linhares, Ulisses C, Andrade, Arnaldo F, Bento, Cleonice A
Format: Article
Language:English
Subjects:
Adolescent
Adult
Allergy and Immunology
Antigens, CD - metabolism
Anxiety Disorders - pathology
Cell Proliferation - drug effects
Cell Survival - drug effects
Cells, Cultured
Cytokines - metabolism
Dopamine
Dopamine - pharmacology
Enzyme-Linked Immunosorbent Assay - methods
Female
Flow Cytometry
Generalized anxiety disorder
Glucocorticoid
Glucocorticoids - metabolism
Humans
IL-10/TGF-β
Male
Mitogens - pharmacology
Neurology
Phenotype
Phytohemagglutinins - pharmacology
Th17 Cells - drug effects
Th17/Th1
Thymidine - metabolism
Tritium - metabolism
Tuberculin - pharmacology
Up-Regulation - drug effects
Young Adult
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Our objective was to evaluate the effect of stress-related dose of dopamine (DA) on the in vitro proliferation and cytokine production in polyclonally-activated T cells from healthy individuals or individuals with generalized anxiety disorder (GAD). Our results demonstrated that cell cultures from GAD group proliferated less following T cell activation, as compared with control group. The addition of DA reduced the proliferative response in cell cultures from healthy but not from GAD individuals. The cytokine profile in GAD individuals revealed Th1 and Th2 deficiencies associated with a dominant Th17 phenotype, which was enhanced by DA. A similar DA-induced immunomodulation was also observed in PPD-activated cell cultures from GAD individuals. Unlike the control, DA-enhanced Th17 cytokine production in GAD individuals was not affected by glucocorticoid. In conclusion, our results show that the T cell functional dysregulation in GAD individuals is significantly amplified by DA. These immune abnormalities can have impact in increasing the susceptibility of individuals with anxiety disorders to infectious diseases and inflammatory/autoimmune disorders.
ISSN:0165-5728
1872-8421
DOI:10.1016/j.jneuroim.2011.06.009