Microfluidic Chromatography for Early Stage Evaluation of Biopharmaceutical Binding and Separation Conditions

Optimization of separation conditions for biopharmaceuticals requires evaluation of a large number of process variables. To miniaturize this evaluation a microfluidic column (1.5 µL volume and 1 cm height) was fabricated and packed with a typical process scale resin. The device was assessed by compa...

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Bibliographic Details
Published in:Separation science and technology 2011-01, Vol.46 (2), p.185-194
Main Authors: Shapiro, Michael S., Haswell, Stephen J., Lye, Gary J., Bracewell, Daniel G.
Format: Article
Language:English
Subjects:
breakthrough
elution
ion exchange
microfluidics
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Summary:Optimization of separation conditions for biopharmaceuticals requires evaluation of a large number of process variables. To miniaturize this evaluation a microfluidic column (1.5 µL volume and 1 cm height) was fabricated and packed with a typical process scale resin. The device was assessed by comparison to a protein separation at conventional laboratory scale. This was based upon measurement of the quality of packing and generation of breakthrough and elution curves. Dynamic binding capacities from the microfluidic column compared well with the laboratory scale. Microfluidic scale gradient elution separations also equated to the laboratory column three orders of magnitude larger in scale.
ISSN:0149-6395
1520-5754
DOI:10.1080/01496395.2010.511641