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Preparation and physical properties of a novel biocompatible porcine corneal acellularized matrix
This study was to investigate the stability, physico-mechanical property and biocompatibility of porcine corneal acellularized matrix (PCAM) that was prepared using human sera treatment to decellularize corneas. The stability (the rate of biodegradation) and physico-mechanical property (water uptake...
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| Published in: | In vitro cellular & developmental biology. Animal 2010-07, Vol.46 (7), p.600-605 |
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| Main Authors: | , , , , , , , , , |
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| cited_by | cdi_FETCH-LOGICAL-c448t-565a021de1de5b3e772e2c711dfae3377dfa05353ad97861cef084b0bba7cc1a3 |
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| cites | cdi_FETCH-LOGICAL-c448t-565a021de1de5b3e772e2c711dfae3377dfa05353ad97861cef084b0bba7cc1a3 |
| container_end_page | 605 |
| container_issue | 7 |
| container_start_page | 600 |
| container_title | In vitro cellular & developmental biology. Animal |
| container_volume | 46 |
| creator | Shao, Yi Quyang, Luowa Zhou, Yueping Tang, Jing Tan, Yuehui Liu, Qiuping Lin, Zhirong Yin, Tingting Qiu, Fangfang Liu, Zuguo |
| description | This study was to investigate the stability, physico-mechanical property and biocompatibility of porcine corneal acellularized matrix (PCAM) that was prepared using human sera treatment to decellularize corneas. The stability (the rate of biodegradation) and physico-mechanical property (water uptake, density, and porosity) of PCAM were not compromised, compared with porcine fresh cornea matrix (PFCM, p > 0.05). The contact and extract cytotoxicity tests with human corneal epithelial cells and human keratocytes showed that PCAM has a good biocompatibility ex vivo and no cytotoxic effect. These results present the ability to create safety scaffolds that function as cornea grafts and provide a novel experimental approach for the study of cornea tissue engineering using acellular porcine cornea. |
| doi_str_mv | 10.1007/s11626-010-9328-9 |
| format | article |
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The stability (the rate of biodegradation) and physico-mechanical property (water uptake, density, and porosity) of PCAM were not compromised, compared with porcine fresh cornea matrix (PFCM, p > 0.05). The contact and extract cytotoxicity tests with human corneal epithelial cells and human keratocytes showed that PCAM has a good biocompatibility ex vivo and no cytotoxic effect. These results present the ability to create safety scaffolds that function as cornea grafts and provide a novel experimental approach for the study of cornea tissue engineering using acellular porcine cornea.</description><identifier>ISSN: 1071-2690</identifier><identifier>EISSN: 1543-706X</identifier><identifier>DOI: 10.1007/s11626-010-9328-9</identifier><identifier>PMID: 20532994</identifier><identifier>CODEN: IVCAED</identifier><language>eng</language><publisher>New York: New York : Springer-Verlag</publisher><subject>Animal Genetics and Genomics ; Animals ; Biocompatibility ; Biocompatible Materials - metabolism ; Biodegradation ; Biomaterials ; Biomedical and Life Sciences ; BIOTECHNOLOGY ; Cell Biology ; Cell Culture ; Cell Proliferation ; Cells, Cultured ; Cornea ; Cornea - cytology ; Cornea - metabolism ; Corneal Stroma - cytology ; Cytotoxicity ; Developmental Biology ; Epithelial cells ; Epithelium, Corneal - cytology ; Extracellular Matrix - metabolism ; Heterologous transplantation ; Humans ; Life Sciences ; Materials Testing ; Porosity ; Scaffolds ; Sincalide - metabolism ; Stem Cells ; Sus scrofa ; Tissue engineering ; Tissue Engineering - methods ; Tissue grafting ; Water - metabolism ; Water uptake</subject><ispartof>In vitro cellular & developmental biology. Animal, 2010-07, Vol.46 (7), p.600-605</ispartof><rights>2010 Society for In Vitro Biology</rights><rights>The Society for In Vitro Biology 2010</rights><rights>Copyright Society for In Vitro Biology Jul 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-565a021de1de5b3e772e2c711dfae3377dfa05353ad97861cef084b0bba7cc1a3</citedby><cites>FETCH-LOGICAL-c448t-565a021de1de5b3e772e2c711dfae3377dfa05353ad97861cef084b0bba7cc1a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/40802404$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/40802404$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,58238,58471</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20532994$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shao, Yi</creatorcontrib><creatorcontrib>Quyang, Luowa</creatorcontrib><creatorcontrib>Zhou, Yueping</creatorcontrib><creatorcontrib>Tang, Jing</creatorcontrib><creatorcontrib>Tan, Yuehui</creatorcontrib><creatorcontrib>Liu, Qiuping</creatorcontrib><creatorcontrib>Lin, Zhirong</creatorcontrib><creatorcontrib>Yin, Tingting</creatorcontrib><creatorcontrib>Qiu, Fangfang</creatorcontrib><creatorcontrib>Liu, Zuguo</creatorcontrib><title>Preparation and physical properties of a novel biocompatible porcine corneal acellularized matrix</title><title>In vitro cellular & developmental biology. Animal</title><addtitle>In Vitro Cell.Dev.Biol.-Animal</addtitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><description>This study was to investigate the stability, physico-mechanical property and biocompatibility of porcine corneal acellularized matrix (PCAM) that was prepared using human sera treatment to decellularize corneas. The stability (the rate of biodegradation) and physico-mechanical property (water uptake, density, and porosity) of PCAM were not compromised, compared with porcine fresh cornea matrix (PFCM, p > 0.05). The contact and extract cytotoxicity tests with human corneal epithelial cells and human keratocytes showed that PCAM has a good biocompatibility ex vivo and no cytotoxic effect. These results present the ability to create safety scaffolds that function as cornea grafts and provide a novel experimental approach for the study of cornea tissue engineering using acellular porcine cornea.</description><subject>Animal Genetics and Genomics</subject><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biocompatible Materials - metabolism</subject><subject>Biodegradation</subject><subject>Biomaterials</subject><subject>Biomedical and Life Sciences</subject><subject>BIOTECHNOLOGY</subject><subject>Cell Biology</subject><subject>Cell Culture</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Cornea</subject><subject>Cornea - cytology</subject><subject>Cornea - metabolism</subject><subject>Corneal Stroma - cytology</subject><subject>Cytotoxicity</subject><subject>Developmental Biology</subject><subject>Epithelial cells</subject><subject>Epithelium, Corneal - cytology</subject><subject>Extracellular Matrix - metabolism</subject><subject>Heterologous transplantation</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Materials Testing</subject><subject>Porosity</subject><subject>Scaffolds</subject><subject>Sincalide - metabolism</subject><subject>Stem Cells</subject><subject>Sus scrofa</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><subject>Tissue grafting</subject><subject>Water - metabolism</subject><subject>Water uptake</subject><issn>1071-2690</issn><issn>1543-706X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqFkV9r1jAUxoM43Hz1A3ihBm-86jxJmqS5HMN_MHCgA-9Cmp7OvLRNTVpx-_TmpXOCFxpCTuD8nicnPIQ8Y3DKAPSbzJjiqgIGlRG8qcwDcsJkLSoN6uvDcgfNKq4MHJPHOe-hLMPUI3LMQQpuTH1C3GXC2SW3hDhRN3V0_naTg3cDnVOcMS0BM409dXSKP3CgbYg-jnPh2wHpHJMPE1If04RF4zwOwzq4FG6xo6NbUvj5hBz1bsj49K7uyNW7t1_OP1QXn95_PD-7qHxdN0sllXTAWYdly1ag1hy514x1vUMhtC61TC2F64xuFPPYQ1O30LZOe8-c2JHXm28Z_PuKebFjyId53IRxzdYAF1JzDv8ltRCqNqbRhXz1F7mPa5rKN6yWioPWUhaIbZBPMeeEvZ1TGF26sQzsISe75WRLTvaQUzl25MWd8dqO2N0rfgdTAL4BubSma0x_Xv6X6_NNtM9LTPemNTTAaziYvtz6vYvWXaeQ7dVnDkwAa5QUjRG_ABk2sXg</recordid><startdate>20100701</startdate><enddate>20100701</enddate><creator>Shao, Yi</creator><creator>Quyang, Luowa</creator><creator>Zhou, Yueping</creator><creator>Tang, Jing</creator><creator>Tan, Yuehui</creator><creator>Liu, Qiuping</creator><creator>Lin, Zhirong</creator><creator>Yin, Tingting</creator><creator>Qiu, Fangfang</creator><creator>Liu, Zuguo</creator><general>New York : Springer-Verlag</general><general>Springer Science + Business Media</general><general>Springer-Verlag</general><general>Society for In Vitro Biology</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>S0X</scope><scope>7X8</scope><scope>7QO</scope></search><sort><creationdate>20100701</creationdate><title>Preparation and physical properties of a novel biocompatible porcine corneal acellularized matrix</title><author>Shao, Yi ; Quyang, Luowa ; Zhou, Yueping ; Tang, Jing ; Tan, Yuehui ; Liu, Qiuping ; Lin, Zhirong ; Yin, Tingting ; Qiu, Fangfang ; Liu, Zuguo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-565a021de1de5b3e772e2c711dfae3377dfa05353ad97861cef084b0bba7cc1a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animal Genetics and Genomics</topic><topic>Animals</topic><topic>Biocompatibility</topic><topic>Biocompatible Materials - 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Animal</jtitle><stitle>In Vitro Cell.Dev.Biol.-Animal</stitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><date>2010-07-01</date><risdate>2010</risdate><volume>46</volume><issue>7</issue><spage>600</spage><epage>605</epage><pages>600-605</pages><issn>1071-2690</issn><eissn>1543-706X</eissn><coden>IVCAED</coden><abstract>This study was to investigate the stability, physico-mechanical property and biocompatibility of porcine corneal acellularized matrix (PCAM) that was prepared using human sera treatment to decellularize corneas. The stability (the rate of biodegradation) and physico-mechanical property (water uptake, density, and porosity) of PCAM were not compromised, compared with porcine fresh cornea matrix (PFCM, p > 0.05). The contact and extract cytotoxicity tests with human corneal epithelial cells and human keratocytes showed that PCAM has a good biocompatibility ex vivo and no cytotoxic effect. These results present the ability to create safety scaffolds that function as cornea grafts and provide a novel experimental approach for the study of cornea tissue engineering using acellular porcine cornea.</abstract><cop>New York</cop><pub>New York : Springer-Verlag</pub><pmid>20532994</pmid><doi>10.1007/s11626-010-9328-9</doi><tpages>6</tpages></addata></record> |
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| ispartof | In vitro cellular & developmental biology. Animal, 2010-07, Vol.46 (7), p.600-605 |
| issn | 1071-2690 1543-706X |
| language | eng |
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| source | Springer Link; JSTOR Archival Journals |
| subjects | Animal Genetics and Genomics Animals Biocompatibility Biocompatible Materials - metabolism Biodegradation Biomaterials Biomedical and Life Sciences BIOTECHNOLOGY Cell Biology Cell Culture Cell Proliferation Cells, Cultured Cornea Cornea - cytology Cornea - metabolism Corneal Stroma - cytology Cytotoxicity Developmental Biology Epithelial cells Epithelium, Corneal - cytology Extracellular Matrix - metabolism Heterologous transplantation Humans Life Sciences Materials Testing Porosity Scaffolds Sincalide - metabolism Stem Cells Sus scrofa Tissue engineering Tissue Engineering - methods Tissue grafting Water - metabolism Water uptake |
| title | Preparation and physical properties of a novel biocompatible porcine corneal acellularized matrix |
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