“DNA-Dressed NAnopore” for complementary sequence detection

Single molecule electrical sensing with nanopores is a rapidly developing field with potential revolutionary effects on bioanalytics and diagnostics. The recent success of this technology is in the simplicity of its working principle, which exploits the conductance modulations induced by the electro...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2011-11, Vol.29 (1), p.125-131
Main Authors: Mussi, Valentina, Fanzio, Paola, Repetto, Luca, Firpo, Giuseppe, Stigliani, Sara, Tonini, Gian Paolo, Valbusa, Ugo
Format: Article
Language:English
Subjects:
Base Sequence
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Chemical functionalization
DNA - genetics
DNA analysis
DNA Probes - genetics
Electric Conductivity
Hybridization
Nanofabrication
Nanopore biosensors
Nanopores - ultrastructure
Nucleic Acid Hybridization
Oligonucleotides - genetics
Single molecule sensing
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Summary:Single molecule electrical sensing with nanopores is a rapidly developing field with potential revolutionary effects on bioanalytics and diagnostics. The recent success of this technology is in the simplicity of its working principle, which exploits the conductance modulations induced by the electrophoretic translocation of molecules through a nanometric channel. Initially proposed as fast and powerful tools for molecular stochastic sensing, nanopores find now application in a range of different domains, thanks to the possibility of finely tuning their surface properties, thus introducing artificial binding and recognition sites. Here we show the results of DNA translocation and hybridization experiments at the single molecule level by a novel class of selective biosensor devices that we call “DNA-Dressed NAnopore” (DNA2), based on solid state nanopore with large initial dimensions, resized and activated by functionalization with DNA molecules. The presented data demonstrate the ability of the DNA2 to selectively detect complementary target sequences, that is to distinguish between molecules depending on their affinity to the functionalization. The DNA2 can thus constitute the basis for the design of integrable parallel devices for mutation DNA analysis, diagnostics and bioanalytic investigations.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2011.08.005