Kinetics and Pathogenicity of Equine Herpesvirus-9 Infection following Intraperitoneal Inoculation in Hamsters

The kinetics of infection and pathogenicity of equine herpesvirus-9 (EHV-9) was studied in a hamster model. Five-week-old Syrian hamsters and 5-day-old suckling hamsters were inoculated intraperitoneally with 10 5 and 4 × 10 4 plaque-forming units of EHV-9, respectively. EHV-9 antigens were detected...

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Bibliographic Details
Published in:Journal of comparative pathology 2011-08, Vol.145 (2), p.271-281
Main Authors: El-Nahass, E., El-Habashi, N., Nayel, M., Kasem, S., Fukushi, H., Suzuki, Y., Hirata, A., Sakai, H., Yanai, T.
Format: Article
Language:English
Subjects:
Animals
Antigens, Viral - analysis
Cricetinae
Disease Models, Animal
DNA, Viral
EHV-9
hamster model
Herpesviridae Infections - pathology
Herpesviridae Infections - virology
Kinetics
Mesocricetus
neuropathogenesis
Polymerase Chain Reaction
Varicellovirus
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Summary:The kinetics of infection and pathogenicity of equine herpesvirus-9 (EHV-9) was studied in a hamster model. Five-week-old Syrian hamsters and 5-day-old suckling hamsters were inoculated intraperitoneally with 10 5 and 4 × 10 4 plaque-forming units of EHV-9, respectively. EHV-9 antigens were detected by immunocytochemistry in the peritoneal macrophages, which may be the primary site of virus attachment and propagation at 6 h post inoculation (hpi). At 12 hpi, viral antigen was observed in the abdominal nerves and ganglia (mainly the coeliac ganglia). Virus antigen was detected in the dorsal root (spinal) ganglia, in parts of the spinal cord (particularly the mid-lumbar area) and in the myenteric plexuses at 36, 48 and 72 hpi, respectively. At 96 hpi, virus antigen was detected in the most caudal part of the brain. Polymerase chain reaction conducted on samples of the blood, spinal cord and brain revealed EHV-9 DNA in the spinal cord at 36 hpi and in the blood at 48 hpi and for 4 days after this initial detection. It is suggested that after initial propagation in the abdominal macrophages, EHV-9 infected the abdominal ganglia or myenteric plexuses and then travelled to the brain via the peripheral nerves and spinal cord. Examination of other organs also revealed the presence of EHV-9, suggesting that the virus might infect tissues other than those of the nervous system.
ISSN:0021-9975
1532-3129
DOI:10.1016/j.jcpa.2011.01.009