Effect of Azospirillum brasilense Sp245 lipopolysaccharide on the functional activity of wheat root meristematic cells

• Published in: Plant and soil 2011-09, Vol.346 (1/2), p.181-188
• Main Authors: Evseeva, Nina V., Matora, Larisa Yu, Burygin, Gennady L., Dmitrienko, Vasily V., Shchyogolev, Sergei Yu
• Format: Article
• Language: English
• Subjects: Anatomy & physiology; Azospirillum; Azospirillum brasilense; Bacteria; Beans; Biochemistry; Biomedical and Life Sciences; Cells; E coli; Ecology; Escherichia coli; Inoculation; Legumes; Life Sciences; Lipopolysaccharides; Mimosaceae; Mitogens; Mitotic index; Physiological aspects; Plant antigens; Plant interaction; Plant Physiology; Plant roots; Plant Sciences; Plants; Regular Article; Rhizobium leguminosarum; Seedlings; Soil Science & Conservation; Triticum aestivum; Wheat
• ISSN: 0032-079X; 1573-5036
• DOI: 10.1007/s11104-011-0808-9

We studied changes in the physiological and biochemical parameters of wheat (Triticum aestivum L. 'Saratovskaya 29') seedlings treated with lipopolysaccharide isolated from the outer membrane of the associative bacterium Azospirillum brasilense Sp245. The obtained data were compared with (i) the results of plant inoculation with whole Sp245 cells and (ii) the effects exerted by the lipopolysaccharide and whole cells of the enterobacterium Escherichia coli K12 and the specific legume symbiont Rhizobium leguminosarum 249. The functional activity of meristematic cells was judged by their mitotic index and by the results of immunochemical determination of the proliferative antigen of initials, a molecular marker for wheat meristem cells. Treatment of the seedling root system with 10 μg mL-1 of Sp245 lipopolysaccharide increased the mitotic index (1.8-fold) and the antigen content (approximately 1.4-fold). These increases were comparable to the effects produced by whole cell inoculation (2- and 1.4-fold, respectively). Our findings give grounds to consider lipopolysaccharide as an active component of the Azospirillum cell surface that not only determines bacterial contact interactions with wheat roots but also participates in the induction of plant responses to these interactions. We finally discuss the linkage between the proliferative antigen of initials and the transduction of a hormonal signal to the cell, as well as the informational value of this antigen as an indicator of effectiveness of plant–bacterial interactions.