Ex vivo cartilage defect model for the evaluation of cartilage regeneration using mesenchymal stem cells

An ex vivo cartilage defect model for the evaluation of cartilage regeneration using mesenchymal stem cells (MSCs) was developed. Porcine chondrocytes and human MSCs were transplanted into cartilage defects created on the porcine osteochondral and chondral discs and cultivated for 3 weeks. Although...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2011-03, Vol.111 (3), p.357-364
Main Authors: Iwai, Ryosuke, Fujiwara, Masashi, Wakitani, Shigeyuki, Takagi, Mutsumi
Format: Article
Language:English
Subjects:
Adult
Animal models
Animals
Biological and medical sciences
Biotechnology
Cartilage
Cartilage - cytology
Cartilage regeneration
Cells, Cultured
Chondrocytes
Chondrocytes - cytology
Culture Media
Cytokine
Explant culture
Fibroblast growth factor 2
Fibroblast Growth Factor 2 - metabolism
Fundamental and applied biological sciences. Psychology
Human mesenchymal stem cell
Humans
Male
Mesenchymal Stem Cell Transplantation
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - metabolism
Mesenchyme
Regeneration
Stem cells
Swine
Tissue Engineering
Transfection
Transforming Growth Factor beta3 - genetics
Transforming Growth Factor beta3 - metabolism
Transforming growth factor- beta
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Summary:An ex vivo cartilage defect model for the evaluation of cartilage regeneration using mesenchymal stem cells (MSCs) was developed. Porcine chondrocytes and human MSCs were transplanted into cartilage defects created on the porcine osteochondral and chondral discs and cultivated for 3 weeks. Although the regeneration of cartilage-like tissues was observed after the transplantation of chondrocytes to defects on both of the osteochondral and chondral discs, the transplanted MSCs formed cartilage-like tissues only in the defect on the chondral disc, in which an in vivo cartilage-like structure was partly observed, and a degraded tissue was observed in the defect on the osteochondral disc. The effects of medium additives such as serum, transforming growth factor-β3 (TGF-β3), and fibroblast growth factor-2, and the transfection of TGF-β3 gene to MSCs could be clearly estimated using the cartilage defect model. In conclusion, a chondral disc with defects is useful for evaluating cartilage regeneration using MSCs.
ISSN:1389-1723
1347-4421
DOI:10.1016/j.jbiosc.2010.11.001