Genetic diversity of Mycobacterium Tuberculosis strains circulating in Kharkiv Oblast, Ukraine

• Published in: Molecular genetics, microbiology and virology microbiology and virology, 2011-03, Vol.26 (1), p.21-26
• Main Authors: Dymova, M. A., Lyashenko, A. A., Poteiko, P. I., Kashuba, D. A., Stadnikova, A. V., Krut’ko, V. S., Filipenko, M. L.
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Experimental Works; Genetic diversity; Life Sciences; Microbiology; Molecular Medicine; Mycobacterium tuberculosis
• ISSN: 0891-4168; 1934-841X
• DOI: 10.3103/S0891416811010022

A molecular genetic study of a tuberculosis mycobacteria isolates obtained from 98 patients with pulmonary tuberculosis residing in the Kharkiv oblast (Ukraine) has been performed for the first time by VNTR and IS6110 RFLP typing methods. Seventy-five different genetic profiles have been identified. Isolates from the Beijing family (34%, 34 isolates) and the LA-M family (23%, 23 isolates) have prevailed. An isolate cluster belonging to the LA-M family has had significant genetic heterogeneity, which indicates the ancient spread and circulation of this family on the studied territory. The strains from the Beijing (31%) and LA-M (14%) families have been mostly spread among the originally detected patients; their frequencies in the group of patients with tuberculosis detected for the second time are 39% and 29%, respectively. An increase in the frequencies of the isolates from these families during chronic tuberculosis have not been statistically significant (χ 2 = 0.33, p = 0.56; χ 2 = 2.08, p = 0.15, respectively). An association between belonging to the LA-M family and the multidrug resistance of isolates ( r = 0.27, p = 0.0059) has been detected. In addition, we have discovered a significant proportion of isolates (36%) that are attributed to yet unidentified families of M. tuberculosis or have individual unclustering genotypes.