Comparative efficacy of conventional primer sets in detection of Cryptosporidium parvum for diagnostic use

In this study, the sensitivity and specificity of different previously described primer sets for Cryptosporidium parvum detection by polymerase chain reaction (PCR) was evaluated. For this purpose, the primer sets defined by Cacciò et al. (FEMS Microbiol Lett 170(1):173-179, 1999) (tub), Widmer et a...

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Bibliographic Details
Published in:Parasitology research (1987) 2010-02, Vol.106 (3), p.683-687
Main Authors: Kar, Sirri, Daugschies, Arwid, Bangoura, Berit
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Cattle
Cattle Diseases - parasitology
Cell culture
Cell Line
Cryptosporidiosis - diagnosis
Cryptosporidiosis - parasitology
Cryptosporidium parvum
Cryptosporidium parvum - genetics
Cryptosporidium parvum - isolation & purification
DNA
DNA Primers - genetics
Feces
Feces - parasitology
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Humans
Immunology
Invertebrates
Medical Microbiology
Microbiology
Molecular Diagnostic Techniques - methods
Oocysts
Original Paper
Parasitology - methods
Polymerase chain reaction
Polymerase Chain Reaction - methods
Primers
Sensitivity and Specificity
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Summary:In this study, the sensitivity and specificity of different previously described primer sets for Cryptosporidium parvum detection by polymerase chain reaction (PCR) was evaluated. For this purpose, the primer sets defined by Cacciò et al. (FEMS Microbiol Lett 170(1):173-179, 1999) (tub), Widmer et al. (Appl Environ Microbiol 64(11):4477-4481, 1998) (btub) and Rochelle et al. (Appl Environ Microbiol 63:2029-2037, 1997) (cphsp), respectively, were used. Deoxyribonucleic acid (DNA) was isolated from three different sample materials: (1) from the faeces of an experimentally C. parvum-infected calf, (2) from purified C. parvum oocysts, and (3) from C. parvum-infected HCT-8 cell cultures. The DNA samples were subjected to PCR reactions with each of the three given primer sets to investigate sensitivity and suitability for routine use. The primers described by Cacciò et al. (FEMS Microbiol Lett 170(1):173-179, 1999) (TUB) were superior regarding sensitivity and specificity in terms of detection of C. parvum in faeces, in purified oocysts and also in cell culture, and may thus be applied for routine diagnostic use in common sample materials.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-010-1737-x