Decomposition of Cr(V)-diols to Cr(III) Complexes by Arthrobacter oxydans

We demonstrated previously that Cr(VI) is readily reduced to oxoCr(V)-diols at the surface of Arthrobacter oxydans--a Gram-positive aerobic bacteria isolated from Columbia basalt rocks originated from a highly contaminated site in the USA. Here, we report an electron spin resonance (ESR) study of Cr...

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Bibliographic Details
Published in:Microbial ecology 2009-02, Vol.57 (2), p.360-366
Main Authors: Tsibakhashvili, Nelly Y, Kalabegishvili, Tamaz L, Rcheulishvili, Alexander N, Murusidze, Ivane G, Rcheulishvili, Olia A, Kerkenjia, Salome M, Holman, Hoi-Ying N
Format: Article
Language:English
Subjects:
Aerobic bacteria
Aerobic conditions
Arthrobacter
Arthrobacter - metabolism
Arthrobacter oxydans
Atomic absorption spectroscopy
Bacteria
Basalt
Biodegradation, Environmental
Biological and medical sciences
Biomedical and Life Sciences
Cell growth
Cell walls
Chromium
Chromium Compounds - metabolism
Decomposition
Ecology
Electron paramagnetic resonance
Electron Spin Resonance Spectroscopy
Fundamental and applied biological sciences. Psychology
Geoecology/Natural Processes
Hydroxides
Kinetics
Life Sciences
Liquids
Microbial Ecology
Microbiology
Models, Biological
Nature Conservation
Original Article
SPECIAL SECTION
Spectral analysis
Spectrometry
Spectrophotometry, Atomic
Time Factors
Toxicity
Water Quality/Water Pollution
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Summary:We demonstrated previously that Cr(VI) is readily reduced to oxoCr(V)-diols at the surface of Arthrobacter oxydans--a Gram-positive aerobic bacteria isolated from Columbia basalt rocks originated from a highly contaminated site in the USA. Here, we report an electron spin resonance (ESR) study of Cr(III) hydroxide formation from Cr(V)-diols by this bacterial strain as cells were exposed to 35, 200, and 400 mg/L of Cr(VI) under aerobic conditions as a batch culture and as lyophilized cells. The time-dependent ESR measurements show that the half-time of Cr(III) formation is almost equal to that of Cr(V) decomposition, which is in the range of 3-6 days for all cases. This rate is at least 300 times slower than that of Cr(V) formation. Additionally, atomic absorption spectrometry was also employed to examine the time course of total chromium in bacterial cells. This is the first time the kinetics of Cr(III) complexes formation in bacteria is evaluated.
ISSN:0095-3628
1432-184X
DOI:10.1007/s00248-008-9476-6