A new way to detect the interaction of DNA and anticancer drugs based on the decreased resonance light scattering signal and its potential application

• Published in: Analyst (London) 2011-10, Vol.136 (19), p.3927-3933
• Main Authors: Chen, Zhanguang, Song, Tianhe, Peng, Yurui, Chen, Xi, Chen, Junhui, Zhang, Guomin, Qian, Sihua
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Chemistry; DNA, Neoplasm - analysis; DNA, Neoplasm - drug effects; Exact sciences and technology; Fluorescent Dyes - chemistry; Humans; Light; Methylene Blue - chemistry; Molecular Structure; Scattering, Radiation; Sensitivity and Specificity; Spectrometric and optical methods
• ISSN: 0003-2654; 1364-5528
• DOI: 10.1039/c1an15507e

A novel assay has been developed to detect the interaction of DNA and anticancer drugs based on the decreased resonance light scattering (RLS) technique. The proposed method can be used to study those drugs which do not produce a RLS-signal after binding to DNA. RLS was used to monitor the interaction of five anticancer drugs with DNA. The reaction between anticancer drugs and DNA took place in BR buffer solution. From the RLS assay, the sequence of five anticancer drugs activities was as follows: CTX < MTX < Pt < MMC < 5-Fu. Mammary cancer cell DNA (mcDNA) was involved to validate the RLS assay. The results showed that the sensitivities of the five anticancer drugs targeting both mcDNA and ctDNA increased in the same order. However the sensitivity of each drug to mcDNA was higher than that to ctDNA It is a significant innovation of the RLS method to detect the interaction of DNA and anticancer drugs and to obtain drug sensitivity, which provides new strategies to screen DNA targeted anticancer drugs.