Molecular characterization of Staphylococcus pseudintermedius strains isolated from clinical samples of animal origin

• Published in: Folia microbiologica 2011-09, Vol.56 (5), p.415-422
• Main Authors: Chrobak, D., Kizerwetter-Świda, M., Rzewuska, M., Moodley, A., Guardabassi, L., Binek, M.
• Format: Article
• Language: English
• Subjects: Animal Diseases - epidemiology; Animal Diseases - microbiology; Animals; Applied Microbiology; Bacteria; Bacterial Proteins - analysis; Bacterial Proteins - genetics; Bacterial Typing Techniques; Biomedical and Life Sciences; Body Fluids - microbiology; Cats; Cattle; Chromosomes; Clinical isolates; Dogs; Drug Resistance, Bacterial; Environmental Engineering/Biotechnology; Genetic diversity; Gerbillinae; Immunology; Life Sciences; Microbiology; Micrococcal Nuclease - analysis; Micrococcal Nuclease - genetics; Molecular biology; Multiplex Polymerase Chain Reaction; Penicillin-Binding Proteins; Poland - epidemiology; Polymerase chain reaction; Polymorphism, Restriction Fragment Length; pta gene; Rabbits; Restriction fragment length polymorphism; RNA, Ribosomal, 16S - analysis; rRNA 16S; Staphylococcal Infections - epidemiology; Staphylococcal Infections - microbiology; Staphylococcal Infections - veterinary; Staphylococcal Protein A - analysis; Staphylococcal Protein A - genetics; Staphylococcus; Staphylococcus intermedius; Staphylococcus intermedius - classification; Staphylococcus intermedius - genetics; Staphylococcus intermedius - isolation & purification; Staphylococcus intermedius - pathogenicity; Typing
• ISSN: 0015-5632; 1874-9356
• DOI: 10.1007/s12223-011-0064-7

The aim of this study was to determine the species distribution among 44 randomly selected clinical isolates (30 mecA -positive and 14 mecA -negative) of animal origin previously identified as Staphylococcus intermedius by phenotypic tests and species-specific PCR amplification of the 16S rRNA gene. For this purpose, we used a multiplex PCR for the detection of the nuc gene and restriction fragment length polymorphism analysis of pta gene amplified by PCR. Both methods allow discrimination of Staphylococcus pseudintermedius from the other closely related members of the S. intermedius group and other coagulase-positive staphylococci isolated from animals. Genetic diversity of S. pseudintermedius strains was analyzed by staphylococcal protein A-encoding gene ( spa ) typing. Multiplex PCR method was used to identify staphylococcal cassette chromosome mec (SCC mec ) type in mecA -positive strains. All isolates previously identified as S. intermedius were shown to belong to S. pseudintermedius . According to PCR-based SCC mec typing, SCC mec III was the most prevalent type ( n  = 23), and solely seven isolates were designated as non-typeable. Furthermore, the assessment of spa -typing results revealed that the majority of all strains ( n  = 27) harbored spa type t02, and 17 strains were classified as non-typeable.