Spectrophotometric Study on the Binding of Two Water Soluble Schiff Base Complexes of Mn (III) with ct-DNA

In this work, binding of two water soluble Schiff base complexes: Bis sodium (5-sulfosalicylaldehyde) o- phenylendiiminato) Manganese (III) acetate (Salophen complex) and Bis sodium (5-sulfosalicylaldehyde) 1, 2 ethylendiiminato) Manganese (III) acetate (Salen complex) with calf thymus (ct) DNA were...

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Bibliographic Details
Published in:Journal of fluorescence 2011-07, Vol.21 (4), p.1649-1658
Main Authors: Dehkordi, Maryam Nejat, Bordbar, Abdol-Khlegh, Mehrgardi, Masood Ayatolahi, Mirkhani, Valiolah
Format: Article
Language:English
Subjects:
Analytical Chemistry
Animals
Binding Sites
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biomedicine
Biophysics
Biotechnology
Cattle
Circular Dichroism
DNA - chemistry
Electrochemistry
Manganese - chemistry
Molecular Structure
Organometallic Compounds - chemistry
Original Paper
Protein Denaturation
Schiff Bases - chemistry
Sodium Chloride - chemistry
Solubility
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Temperature
Viscosity
Water - chemistry
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Summary:In this work, binding of two water soluble Schiff base complexes: Bis sodium (5-sulfosalicylaldehyde) o- phenylendiiminato) Manganese (III) acetate (Salophen complex) and Bis sodium (5-sulfosalicylaldehyde) 1, 2 ethylendiiminato) Manganese (III) acetate (Salen complex) with calf thymus (ct) DNA were investigated by using different spectroscopic and electrometric techniques including UV-vis, Circular dichroism (CD) and fluorescence spectroscopy, viscommetry and cyclic voltammetry (CV). Both complexes have shown a hyperchromic and a small bathochromic shift in the visible region spectra. A competitive binding study showed that the enhanced emission intensity of ethidium bromide (EB) in the presence of DNA was quenched by the addition of the two Schiff base complexes indicating that they displace EB from its binding site in DNA. Moreover structural changes in the CD spectra and an increase in the CV spectra with addition of DNA were observed. The results show that both complexes bind to DNA. The binding constants have been calculated using fluorescence data for two complexes also K b was calculated with fluorescence Scatchard plot for Salophen. Ultimately, the experimental results show that the dominant interactions are electrostatic while binding mode is surface binding then followed by hydrophobic interactions in grooves in high concentration of complexes.
ISSN:1053-0509
1573-4994
DOI:10.1007/s10895-011-0854-y