A novel amperometric biosensor for oxalate determination using multi-walled carbon nanotube-gold nanoparticle composite

An amperometric oxalate biosensor using nanohybrid film of multi-walled carbon nanotubes (MWCNTs) and gold colloidal nanoparticles (GNPs) via carbodiimide chemistry by forming amide linkages between carboxylic acid groups on the CNTs and amine residues of cysteamine self-assembled monolayer (SAM) ha...

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Published in:Sensors and actuators. B, Chemical Chemical, 2011-07, Vol.155 (2), p.796-803
Main Authors: Pundir, C.S., Chauhan, Nidhi, Rajneesh, Verma, Manjeet, Ravi
Format: Article
Language:English
Subjects:
Amperometric biosensor
biosensors
blood serum
carbon
carbon nanotubes
chlorides
cysteamine
detection limit
Electrical measurements
electrochemistry
Electrodes
foods
Fourier transform infrared spectroscopy
Gold
Gold nanoparticles
grain sorghum
Multi-walled carbon nanotubes
Nanocomposites
Nanomaterials
nanoparticles
Nanostructure
nitrates
Oxalate
Oxalate oxidase
Oxalates
Oxalic acid
scanning electron microscopy
Sorghum
temperature
urine
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Summary:An amperometric oxalate biosensor using nanohybrid film of multi-walled carbon nanotubes (MWCNTs) and gold colloidal nanoparticles (GNPs) via carbodiimide chemistry by forming amide linkages between carboxylic acid groups on the CNTs and amine residues of cysteamine self-assembled monolayer (SAM) has been prepared. The c-MWCNTs were immobilized on the gold (Au) electrode and characterized by FTIR. The morphologies of the c-MWCNT/Au and GNPs/MWCNT/Au electrodes were investigated by scanning electron microscopy (SEM) and the electrochemical performance of the Au, c-MWCNT/Au and GNPs/c-MWCNT/Au electrodes were also studied amperometrically. The Cl − and NO 3 − insensitive oxalate oxidase from grain sorghum was finally immobilized on this electrode. The influence of pH, temperature and oxalate concentration on electrode activity was studied. The electrode showed optimum response within 7 s. The electrocatalytic response showed a linear dependence on the oxalic acid concentration ranging from 1 to 800 μM with a detection limit of 1 μM. The K m value for the oxalic acid sensor was 444.44 μM. The enzyme electrode retained 30% of its initial activity after 5 months, when stored at 4 °C. The electrode was employed for measurement of oxalic acid in serum, urine and foodstuffs.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2011.01.050