The Influence of Hypoxia and Fibrinogen Variants on the Expansion and Differentiation of Adipose Tissue-Derived Mesenchymal Stem Cells

Upon implantation of tissue-engineered scaffolds, hypoxia will occur until neovascularization takes place. In vivo , the temporary fibrin matrix forms a suitable matrix for this process and fibrin variants can influence the extent of neovascularization. In this study, the influence of oxygen tension...

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Bibliographic Details
Published in:Tissue engineering. Part A 2011-11, Vol.17 (21-22), p.2675-2685
Main Authors: Weijers, Ester M., Van Den Broek, Lenie J., Waaijman, Taco, Van Hinsbergh, Victor W.M., Gibbs, Susan, Koolwijk, Pieter
Format: Article
Language:English
Subjects:
Adipose Tissue - cytology
Adipose tissues
Adult
Body fat
Care and treatment
Cell differentiation
Cell Differentiation - genetics
Cell Differentiation - physiology
Cell Hypoxia - physiology
Cell Proliferation - drug effects
Cells, Cultured
Female
Fibrinogen
Fibrinogen - pharmacology
Flow Cytometry
Genetic aspects
Genetic variation
Growth
Humans
Hypoxia
Identification and classification
Leukocytes
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - drug effects
Mesenchymal Stromal Cells - metabolism
Middle Aged
Original Articles
Polymerase Chain Reaction
Properties
Stem cells
Telomere - genetics
Tissue engineering
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Summary:Upon implantation of tissue-engineered scaffolds, hypoxia will occur until neovascularization takes place. In vivo , the temporary fibrin matrix forms a suitable matrix for this process and fibrin variants can influence the extent of neovascularization. In this study, the influence of oxygen tension and naturally occurring fibrinogen variants on adipose tissue-derived mesenchymal stem cell (ASC) expansion and differentiation were determined. ASC proliferated 1.7-fold faster in 1% oxygen and showed reduced cell aging, and their stemness was preserved. The stem cell surface marker expression was similar in 1% and 20% oxygen. The various fibrinogen coatings did not influence ASC expansion and differentiation. Differentiation of ASC toward adipogenic and osteogenic lineages was improved in 20% oxygen, whereas 1% oxygen improved chondrogenic differentiation. In conclusion, optimal oxygen concentrations vary for the intended ASC application, and fibrinogen variants, which can be used to influence neovascularization, do not alter ASC behavior. These data emphasize the importance of oxygen concentrations during stem cell growth and differentiation.
ISSN:1937-3341
1937-335X
DOI:10.1089/ten.tea.2010.0661