Validating the Sensitivity and Performance of Near-Infrared Fluorescence Imaging and Tomography Devices Using a Novel Solid Phantom and Measurement Approach

With the aid of indocyanine green (ICG), lymphatic architecture and function in both mice and humans has been successfully imaged non-invasively using near-infrared (NIR) fluorescence imaging devices. Maximal measurement sensitivity of NIR fluorescence imaging devices is needed for “first-in-humans”...

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Bibliographic Details
Published in:Technology in cancer research & treatment 2012-02, Vol.11 (1), p.95-104
Main Authors: Zhu, B., Tan, I.-C., Rasmussen, J. C., Sevick-Muraca, E. M.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blood and lymphatic vessels
Cardiology. Vascular system
Diagnostic Imaging - instrumentation
Diagnostic Imaging - methods
Diseases of the lymphatic vessels
Fluorescent Dyes
Humans
Indocyanine Green
Lymphedema - diagnosis
Medical sciences
Mice
Phantoms, Imaging
Sensitivity and Specificity
Spectrometry, Fluorescence
Spectroscopy, Near-Infrared
Tomography, X-Ray Computed
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Summary:With the aid of indocyanine green (ICG), lymphatic architecture and function in both mice and humans has been successfully imaged non-invasively using near-infrared (NIR) fluorescence imaging devices. Maximal measurement sensitivity of NIR fluorescence imaging devices is needed for “first-in-humans” molecularly targeting NIR fluorescence agents that are brighter than non-specific ICG. In this study, we developed a solid phantom and measurement approach for the quantification of excitation light leakage and measurement sensitivity of NIR fluorescence imaging devices. The constructed solid phantom, consisting of quantum dots impregnated onto specularly reflective surface, shows long-term stability and can be used as a traceable fluorescence standard. With the constructed solid phantom, the intensified CCD (ICCD)-based device demonstrated more than 300% higher measurement sensitivity compared to the Electron Multiplying CCD (EMCCD) based device when integration time was maintained less than 1.0 s.
ISSN:1533-0346
1533-0338
DOI:10.7785/tcrt.2012.500238