Gender effect on in vitro lymphocyte subset levels of healthy individuals

► There are very few papers that report on the changes in percentage of lymphocyte subsets of PBMC in culture. ► Percentages of B and T cells are significantly higher in cultures from samples of healthy female individuals. ► Percentage of NK cells is significantly higher in cultures from samples of...

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Published in:Cellular immunology 2012, Vol.272 (2), p.214-219
Main Authors: Abdullah, Maha, Chai, Pei-Shin, Chong, Mun-Yee, Tohit, Eusni Rahayu Mohd, Ramasamy, Rajesh, Pei, Chong Pei, Vidyadaran, Sharmili
Format: Article
Language:English
Subjects:
Adult
B-Lymphocytes - immunology
Blood Cell Count - methods
CD3 Complex - immunology
Cells, Cultured
Female
Gender
Healthy individuals
Humans
In vitro
In vivo
Killer Cells, Natural - immunology
Leukocytes, Mononuclear - immunology
Lymphocyte Activation - immunology
Lymphocyte Subsets - immunology
Male
PBMC
Phenotype
Phytohemagglutinins - immunology
Sex Factors
T-Lymphocytes - immunology
Whole blood
Young Adult
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Summary:► There are very few papers that report on the changes in percentage of lymphocyte subsets of PBMC in culture. ► Percentages of B and T cells are significantly higher in cultures from samples of healthy female individuals. ► Percentage of NK cells is significantly higher in cultures from samples of healthy male individuals. ► Phytohaemagglunin significantly changes the profile of PBMC in culture. Differences in gender immune response have resulted in differences in immune protection and susceptibility to inflammatory diseases. Cultured peripheral blood mononuclear cells (PBMC) are widely used in immunomodulation studies, yet the influence of gender is usually not considered. We examined the effect of in vitro culture and phytohaemagglutinin (PHA) stimulation on PBMC lymphocyte subsets using flowcytometry. Full blood counts of whole blood showed higher levels of lymphocyte in male subjects. Lymphocyte subsets enumeration revealed higher NK cell counts in males and higher B cells in females. Cultured PBMC resulted in significant increases in B and total T cell percentages among females and NK cells among males. PHA stimulated significantly increased percentages of NK and total T cells in males and total activated T cells (CD69+) in females. Our results showed significant gender differences in lymphocyte subsets in cultured conditions. This may affect experimental outcome.
ISSN:0008-8749
1090-2163
DOI:10.1016/j.cellimm.2011.10.009