The role of silicon in osteoblast-like cell proliferation and apoptosis

The optimal concentration at which Si induces cell functions has not been fully elucidated. In the present study the effects of Si concentration (0–6 mM) on the biological functions of MG63 cells were investigated. Cell proliferation in the presence of 2 mM Si- and 4 mM Si-containing media progressi...

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Bibliographic Details
Published in:Acta biomaterialia 2011-06, Vol.7 (6), p.2604-2614
Main Authors: Shie, Ming-You, Ding, Shinn-Jyh, Chang, Hsien-Chang
Format: Article
Language:English
Subjects:
Apoptosis
Apoptosis - drug effects
Base Sequence
Blotting, Western
calcium
Calcium silicate
Cell Cycle
Cell Line
Cell proliferation
Cell Proliferation - drug effects
Cell viability
collagen
Culture Media
cultured cells
DNA Primers
Fluorescent Antibody Technique
gene expression
Humans
mitogen-activated protein kinase
Osteoblasts - cytology
Osteoblasts - drug effects
Reverse Transcriptase Polymerase Chain Reaction
secretion
Silicon
Silicon - pharmacology
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Summary:The optimal concentration at which Si induces cell functions has not been fully elucidated. In the present study the effects of Si concentration (0–6 mM) on the biological functions of MG63 cells were investigated. Cell proliferation in the presence of 2 mM Si- and 4 mM Si-containing media progressively increased with culture time, whereas that of 6 mM Si treated MG63 cells was significantly ( P < 0.05) reduced. The unusually high Si concentration (6 mM) induced a significant ( P < 0.05) increase in the sub-G1 phase of cells from the original 3.60% up to 43.01% after culture for 12 h. In contrast, the other lower Si concentration treated MG63 cells in the sub-G1 phase were in the range 3–5% at all culture time points. 4 mM Si treated MG63 cells, but not 6 mM Si treated MG63 cells, showed remarkably enhanced collagen type I (COL I) gene expression and extracellular signal-regulated kinase (ERK) secretion, which were significantly ( P < 0.05) higher than those in the control medium. The activation of ERK was also stimulated in MG63 cells by 4 mM Si. Cells cultured in the presence of 4 mM Si were found to have calcium matrix formation on day 7 that was 15-fold greater than that in the control medium. The results obtained in this study may be useful in designing calcium silicate-based materials with optimal biological properties.
ISSN:1742-7061
1878-7568
DOI:10.1016/j.actbio.2011.02.023