The effect of BMP-2 on micro- and macroscale osteointegration of biphasic calcium phosphate scaffolds with multiscale porosity

• Published in: Acta biomaterialia 2010-08, Vol.6 (8), p.3283-3291
• Main Authors: Lan Levengood, Sheeny K., Polak, Samantha J., Poellmann, Michael J., Hoelzle, David J., Maki, Aaron J., Clark, Sherrie G., Wheeler, Matthew B., Wagoner Johnson, Amy J.
• Format: Article
• Language: English
• Subjects: Animals; Bone and Bones - cytology; Bone and Bones - diagnostic imaging; Bone and Bones - drug effects; Bone ingrowth; Bone Morphogenetic Protein 2 - pharmacology; Calcium phosphate; Calcium Phosphates - pharmacology; Cell Count; Gelatin - pharmacology; Humans; Microscopy, Electron, Scanning; Microspheres; Organ Size - drug effects; Osseointegration - drug effects; Osteogenesis - drug effects; Osteointegration; Porosity; Porosity - drug effects; Robotics; Scaffold; Sus scrofa; Tissue Scaffolds - chemistry; Tomography, X-Ray Computed
• ISSN: 1742-7061; 1878-7568
• DOI: 10.1016/j.actbio.2010.02.026

It is well established that scaffolds for applications in bone tissue engineering require interconnected pores on the order of 100μm for bone in growth and nutrient and waste transport. As a result, most studies have focused on scaffold macroporosity (>100μm). More recently researchers have investigated the role of microporosity in calcium phosphate -based scaffolds. Osteointegration into macropores improves when scaffold rods or struts contain micropores, typically defined as pores less than ∼50μm. We recently demonstrated multiscale osteointegration, or growth into both macropores and intra-red micropores (