Molecular cloning and functional characterization of the porcine extracellular domain of Receptor Activator of NF-κB Ligand (sRANKL)

Receptor Activator of NF-κB Ligand (RANKL) plays a pivotal role as a regulator of osteoclast activity and is involved in osteoporosis. Here, we report the cloning and functional characterization of the complete extracellular domain of the porcine RANKL gene (sRANKL). The porcine sRANKL cDNA has an O...

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Bibliographic Details
Published in:Gene 2012-01, Vol.492 (1), p.296-304
Main Authors: Böcker, Wolfgang, Radic, Tamara, Schönitzer, Veronika, Haasters, Florian, Mutschler, Wolf, Schieker, Matthias
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acids
animal models
Animals
carbonate dehydratase
Cells, Cultured
Cloning
Cloning, Molecular
complementary DNA
Extracellular Space
genes
humans
Lentivirus
mice
molecular cloning
Molecular Sequence Data
nucleotides
Open Reading Frames
osteoclasts
Osteoclasts - cytology
osteoporosis
Porcine
RANK Ligand - genetics
RANK Ligand - metabolism
RANKL
rats
Sequence Alignment
swine
Swine - metabolism
transcription factor NF-kappa B
Up-Regulation
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Summary:Receptor Activator of NF-κB Ligand (RANKL) plays a pivotal role as a regulator of osteoclast activity and is involved in osteoporosis. Here, we report the cloning and functional characterization of the complete extracellular domain of the porcine RANKL gene (sRANKL). The porcine sRANKL cDNA has an ORF of 744 nucleotides and shares 87%, 80% and 80% identity with human, rat and mouse RANKL coding sequences, respectively. The protein consists of 247 amino acids with 90%, 81% and 80% sequences similarities compared to human, mouse and rat RANKL, respectively. Over-expression of porcine sRANKL led to osteoclast formation. The osteoclasts showed a characteristic morphology, expressed the carbonic anhydrase type 2, were TRACP positive and exhibited a bone-resorbing activity. In conclusion, we first describe the molecular cloning and functional characterization of porcine sRANKL, which will help to understand the function of a RANKL gene in large animal models.
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2011.08.008