Distribution of antibiotic resistance in urban watershed in Japan

• Published in: Environmental pollution (1987) 2012-03, Vol.162, p.98-103
• Main Authors: Ham, Young-Sik, Kobori, Hiromi, Kang, Joo-Hyon, Matsuzaki, Takayuki, Iino, Michiyo, Nomura, Hayashi
• Format: Article
• Language: English
• Subjects: Air. Soil. Water. Waste. Feeding; Anti-Bacterial Agents - analysis; Anti-Bacterial Agents - pharmacology; antibiotic resistance; Antibiotic-resistant Escherichia coli; Antibiotics; Applied sciences; Assessments; Biological and medical sciences; Continental surface waters; correlation; Drug Resistance, Bacterial; Environment. Living conditions; Environmental Monitoring; Environmental Protection Agency; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - isolation & purification; Estimating; Exact sciences and technology; Freshwater; Indicators; Japan; Medical sciences; Natural water pollution; Pollution; public health; Public health. Hygiene; Public health. Hygiene-occupational medicine; Resistance diffusion; risk; River basins; Rivers - chemistry; Rivers - microbiology; Spatiotemporal variation; temporal variation; Urban watershed; Water Pollutants, Chemical - analysis; Water Pollutants, Chemical - pharmacology; Water Quality; Water quality assessment; Water treatment and pollution; Watersheds
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/j.envpol.2011.11.002

Antibiotic-resistant E. coli concentrations showed large spatial and temporal variations, with greater concentrations observed in tributaries and downstream than in the upstream and midstream. Twenty percent of the geometric mean concentrations of antibiotic-resistant E. coli in the Tama River basin (Japan) exceeded the maximum acceptable concentration of indicator E. coli established by the USEPA. The indicator E. coli concentrations were positively correlated with those of antibiotic-resistant E. coli and multiple-antibiotic-resistant E. coli (resistance to more than two kinds of antibiotics), respectively, but not the detection rate of antibiotic-resistant E. coli, implying that use of antibiotic-resistant E. coli concentration rather than the detection rate can be a better approach for water quality assessment. Multiple-antibiotic-resistant E. coli is a useful indicator for estimating the resistance diffusion, water quality degradation and public health risk potential. This assessment provides beneficial information for setting national regulatory or environmental standards and managing integrated watershed areas. ► We extensively observed antibiotic-resistant E. coli (AR Ec) in Tama River (Japan). ► AR Ec count rather than the detection rate is better approach for water quality test. ► Multiple-AR Ec is resistant to the antibiotic to which single-AR Ec has no resistance. ► Multiple-AR Ec increase will accelerate the diffusion of antibiotic resistance. Multiple-antibiotic-resistant E. coli in the watershed can cause the diffusion of conventionally rare antibiotic resistance.