Use of Autologous Chondrocytes and Bioinert Perforated Chambers to Tissue Engineer Cartilage In Vivo

Objective To explore the potential applications of a chamber for in vivo tissue engineering, and to establish a novel model for in vivo tissue-engineered cartilage. Methods Four experimental groups were included in this study: (A) chambers + chondrocytes/collagen gel; (B) chambers + chondrocytes/PLG...

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Bibliographic Details
Published in:The Journal of surgical research 2012-03, Vol.173 (1), p.e27-e32
Main Authors: Jiang, Jiang, Ph.D, Li, Jianxue, Ph.D, Hao, Xiaoyan, Ph.D, Diao, Jiansheng, Ph.D, Liu, Bei, Ph.D, Xia, Wei, Ph.D, Guo, Shuzhong, Ph.D
Format: Article
Language:English
Subjects:
Animals
autologous chondrocyte
bioinert perforated chamber
Cartilage - cytology
Cartilage - metabolism
Cell Proliferation
Chondrocytes - cytology
Chondrocytes - metabolism
Collagen - metabolism
Gels - metabolism
in vivo tissue engineered cartilage
Lactic Acid - metabolism
Male
Models, Animal
Polyglycolic Acid - metabolism
Rabbits
Surgery
Tissue Engineering - instrumentation
Tissue Engineering - methods
Transplantation, Autologous
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Summary:Objective To explore the potential applications of a chamber for in vivo tissue engineering, and to establish a novel model for in vivo tissue-engineered cartilage. Methods Four experimental groups were included in this study: (A) chambers + chondrocytes/collagen gel; (B) chambers + chondrocytes/PLGA gel; (C) chondrocytes/collagen gel alone; and (D) chondrocytes/PLGA gel alone. Groups C and D served as controls. The samples were implanted subcutaneously in the donor rabbit, and the contents were harvested at 8 wk after implantation. Results Histologic and immunohistochemical staining and RT-PCR results revealed regenerated cartilage-like tissue in group B and small, irregularly shaped islands of opalescent tissue in group A. In contrast, the control groups displayed vascular invasion and inflammatory reaction, which eventually led to fibrosis and absorption. Conclusions Reproduced cartilages were obtained in an immunocompetent animal model through the use of a bioinert perforated chamber.
ISSN:0022-4804
1095-8673
DOI:10.1016/j.jss.2011.09.041