The movement of self-assembled amphiphilic polymeric nanoparticles in the vitreous and retina after intravitreal injection

Abstract The purpose of this study is to determine the correlation between the distribution of nanoparticles in the vitreous and retina and their surface properties after intravitreal injection. For this purpose, we synthesized seven kinds of nanoparticles through self-assembly of amphiphilic polyme...

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Bibliographic Details
Published in:Biomaterials 2012-04, Vol.33 (12), p.3485-3493
Main Authors: Koo, Heebeom, Moon, Hyungwon, Han, Hyounkoo, Na, Jin Hee, Huh, Myung Sook, Park, Jae Hyung, Woo, Se Joon, Park, Kyu Hyung, Chan Kwon, Ick, Kim, Kwangmeyung, Kim, Hyuncheol
Format: Article
Language:English
Subjects:
Advanced Basic Science
Animals
Dentistry
Drug delivery
Drug Delivery Systems
Intravitreal
Intravitreal Injections
Nanoparticle
Nanoparticles - administration & dosage
Nanoparticles - analysis
Nanoparticles - ultrastructure
Ocular
Rats
Rats, Long-Evans
Retina
Retina - metabolism
Retina - ultrastructure
Surface Properties
Vitreous
Vitreous Body - metabolism
Vitreous Body - ultrastructure
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Summary:Abstract The purpose of this study is to determine the correlation between the distribution of nanoparticles in the vitreous and retina and their surface properties after intravitreal injection. For this purpose, we synthesized seven kinds of nanoparticles through self-assembly of amphiphilic polymer conjugates in aqueous condition. They showed similar size but different surface properties. They were labeled with fluorescent dyes for efficient tracking. After intravitreal injection of these nanoparticles into a rodent eye, their time-dependent distribution in the vitreous and retina was determined in stacking tissue images by confocal microscopy. The results demonstrated that the surface property of nanoparticles is a key factor in determining their distribution in the vitreous and retina after intravitreal injection. In addition, immunohistochemistry and TEM images of retina tissues suggested the important mechanism related with Mülller cells for intravitreally administered nanoparticles to overcome the physical barrier of inner limiting membrane and to penetrate into the deeper retinal structures. Therefore, we expect that this study can provide valuable information for biomedical researchers to develop optimized nanoparticles as drug or gene carriers for retinal and optic nerve disorders such as glaucoma, age-related macular degeneration, and diabetic retinopathy.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2012.01.030