Collagen scaffolds derived from fresh water fish origin and their biocompatibility

• Published in: Journal of biomedical materials research. Part A 2012-04, Vol.100A (4), p.1068-1079
• Main Authors: Pati, Falguni, Datta, Pallab, Adhikari, Basudam, Dhara, Santanu, Ghosh, Kuntal, Mohapatra, Pradeep Kumar Das
• Format: Article
• Language: English
• Subjects: Animals; biocompatibility; Biocompatible Materials; Biological and medical sciences; Biotechnology; collagen; Collagen - chemistry; Female; Fishes; Fresh Water; fresh water fish scale; Fundamental and applied biological sciences. Psychology; Health. Pharmaceutical industry; immunological response; Industrial applications and implications. Economical aspects; Male; Medical sciences; Mice; Mice, Inbred BALB C; Microscopy, Electron, Scanning; Microscopy, Fluorescence; Miscellaneous; nano/micro fibrous scaffold; Spectroscopy, Fourier Transform Infrared; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.33280

Collagen, a major component of native extracellular matrix, has diverse biomedical applications. However, its application is limited due to lack of cost‐effective production and risk of disease transmission from bovine sources currently utilized. This study describes fabrication and characterization of nano/micro fibrous scaffolds utilizing collagen extracted from fresh water fish origin. This is the first time collagen extracted from fresh water fish origin was studied for their biocompatibility and immunogenicity. The nano/micro fibrous collagen scaffolds were fabricated through self‐assembly owing to its amphiphilic nature and were subsequently cross‐linked. In vitro degradation study revealed higher stability of the cross‐linked scaffolds with only ∼50% reduction of mass in 30 days, while the uncross‐linked one degraded completely in 4 days. Further, minimal inflammatory response was observed when collagen solution was injected in mice with or without adjuvant, without significant dilution of sera. The fish collagen scaffolds exhibited considerable cell viability and were comparable with that of bovine collagen. SEM and fluorescence microscopic analysis revealed significant proliferation rate of cells on the scaffolds and within 5 days the cells were fully confluent. These findings indicated that fish collagen scaffolds derived from fresh water origin were highly biocompatible in nature. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.