Molecular identification of unilocular hydatid cysts from domestic ungulates in Ethiopia: Implications for human infections

Abstract To identify the etiologic agents of cystic echinococcosis in Ethiopia, unilocular hydatid cysts were collected from 11 sheep, 16 cattle and 16 camels slaughtered in abattoirs of Aweday, Jijiga, Haramaya and Addis Ababa during June 2010 to February 2011. A PCR-based DNA sequencing of the mit...

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Bibliographic Details
Published in:Parasitology international 2012-06, Vol.61 (2), p.375-377
Main Authors: Hailemariam, Zerihun, Nakao, Minoru, Menkir, Sissay, Lavikainen, Antti, Yanagida, Tetsuya, Okamoto, Munehiro, Ito, Akira
Format: Article
Language:English
Subjects:
Abattoirs
Animals
Base Sequence
camels
Camelus - parasitology
Cattle
Cattle Diseases - parasitology
cytochrome-c oxidase
echinococcosis
Echinococcosis - parasitology
Echinococcosis - veterinary
Echinococcus - genetics
Echinococcus - isolation & purification
Echinococcus canadensis
Echinococcus granulosus
Echinococcus granulosus - genetics
Echinococcus granulosus - isolation & purification
Ethiopia
Gastroenterology and Hepatology
genes
Haplotypes
human diseases
Humans
hydatids
Infectious Disease
Mitochondrial haplotypes
Molecular epidemiology
Molecular Sequence Data
parasitology
polymerase chain reaction
Public Health
sequence analysis
Sequence Analysis, DNA
Sheep
Sheep Diseases - parasitology
slaughterhouses
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Summary:Abstract To identify the etiologic agents of cystic echinococcosis in Ethiopia, unilocular hydatid cysts were collected from 11 sheep, 16 cattle and 16 camels slaughtered in abattoirs of Aweday, Jijiga, Haramaya and Addis Ababa during June 2010 to February 2011. A PCR-based DNA sequencing of the mitochondrial cytochrome oxidase c subunit 1 gene ( cox1 ) was conducted for 40 cysts. The majority of cysts (87.5%) were identified as Echinococcus granulosus sensu stricto and the rest as Echinococcus canadensis . The fertile cysts of E. granulosus s.s. were found only from sheep, although it occurred in all the host species. The predominance of E. granulosus s.s. has important implications for public health since this species is the most typical causative agent of human cystic echinococcosis worldwide. The major cox1 haplotype of E. granulosus s.s. detected in Ethiopia was the same as that has been reported to be most common in Peru and China. However, a few cox1 haplotypes unique to Ethiopia were found in both of the two Echinococcus species. The present regional data would serve as baseline information in determining the local transmission patterns and in designing appropriate control strategies.
ISSN:1383-5769
1873-0329
DOI:10.1016/j.parint.2012.01.007