Suprageneric systematics of flea beetles (Chrysomelidae: Alticinae) inferred from multilocus sequence data

• Published in: Molecular phylogenetics and evolution 2012-03, Vol.62 (3), p.793-805
• Main Authors: Ge, Deyan, Gómez-Zurita, Jesús, Chesters, Douglas, Yang, Xingke, Vogler, Alfried P.
• Format: Article
• Language: English
• Subjects: Altica; Alticinae; Animals; Aphthona; Bayesian analysis; Blepharida; Chaetocnema; Chrysomelidae; Coleoptera - classification; Coleoptera - genetics; Data partitioning; Disonycha; DNA; DNA, Mitochondrial; Evolution, Molecular; Galerucinae; genes; Genes, rRNA; Longitarsus; Metafemoral spring; Multilocus Sequence Typing; nucleotide sequences; paraphyly; Phylogeny; ribosomal RNA; Taxonomic classification
• ISSN: 1055-7903; 1095-9513
• DOI: 10.1016/j.ympev.2011.11.028

[Display omitted] ► Phylogenetic analysis using mitochondrial (rrnL, coxI) and nuclear (SSU, LSU) genes. ► A sequence-based taxonomic classification of Alticinae to define 18 groups of genera. ► Five groups of genera bearing metafemoral springs transferred to Galerucinae. ► Groups of genera diagnosable by unique combinations of morphological traits. Recent phylogenetic studies of flea beetles (Alticinae) based on morphological or molecular data have focused on the relationship and possible paraphyly with respect to the closely related Galerucinae, while the supra-generic classification mainly dates back to the 19th century. Here, phylogenetic analysis was performed on DNA sequences for two mitochondrial (rrnL and cox1) and two nuclear (SSU and LSU rRNA) genes from 158 genera and 165 species that cover most suprageneric groups of flea beetles proposed in the older literature. Various alignment strategies and tree search methods were used to test the stability of major clades. Besides confirmation of the placement of several alticine lineages within Galerucinae, a preliminary framework for classification of the main alticine clades was obtained. It is proposed to recognize 18 groups of genera based on well-supported nodes. These include the Altica, Amphimela, Aphthona, Blepharida, Chabria, Chaetocnema, Dibolia, Disonycha, Griva, Lactica, Longitarsus, Manobia, Monoplatus, Nisotra, Oedionychis, Pentamesa, Phygasia and Pseudodera groups. These groups provide a novel perspective to the existing classification. The analysis of 14 morphological characters used in the traditional classification of Alticinae and Galerucinae revealed high levels of homoplasy with respect to the DNA-based tree, but significant hierarchical structure in most of them. Even if not unique to any particular group of genera, these traits largely corroborate the groupings established with DNA sequences.