Cyclic digene system as a control element of a bacterial biosensor

The Escherichia coli JC158(pCIA12/pGFK5) strain carrying a cyclic digene system with a negative feedback on the pCIA12 plasmid reacting to the DNA damage by changing the synthesis level of reporter genes—GFP and β-galactosidase—was tested. The acquired phenotype was inherited by the next generations...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2012, Vol.48 (1), p.12-16
Main Authors: Stupak, E. E, Stupak, I. V
Format: Article
Language:English
Subjects:
beta-galactosidase
Biochemistry
Biomedical and Life Sciences
Biosensors
Deoxyribonucleic acid
DNA
DNA damage
E coli
Escherichia coli
Life Sciences
Medical Microbiology
Microbiology
phenotype
Plasmids
reporter genes
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Summary:The Escherichia coli JC158(pCIA12/pGFK5) strain carrying a cyclic digene system with a negative feedback on the pCIA12 plasmid reacting to the DNA damage by changing the synthesis level of reporter genes—GFP and β-galactosidase—was tested. The acquired phenotype was inherited by the next generations after the removal of the genotoxic action when the concentration of the DNA-damaging compounds was above the threshold level. A potential has been shown for the application of bacterial biosensors to monitor the presence of genotoxicants in the environment and to test the consequences of short-term exposures to toxic compounds.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683812010164