Abundance of sulphate-reducing bacteria in fish farm sediments along the coast of Japan and South Korea

• Published in: Fisheries science 2012, Vol.78 (1), p.123-131
• Main Authors: Kondo, Ryuji, Shigematsu, Kotaro, Kawahara, Naoki, Okamura, Takahiko, Yoon, Yang Ho, Sakami, Tomoko, Yokoyama, Hisashi, Koizumi, Yoshitsugu
• Format: Article
• Language: English
• Subjects: Aquaculture; Bacteria; Biomedical and Life Sciences; Carbon; Chemical oxygen demand; Cloning; Coasts; Cod; Deep sea; Environmental assessment; Eutrophication; Farms; Fish & Wildlife Biology & Management; Fish farms; Fisheries; Fisheries management; Food Science; Freshwater; Freshwater & Marine Ecology; Indicator organisms; Investigations; Libraries; Life Sciences; Marine fish; Marine pollution; Marine sediments; Organic matter; Original Article; Particulate organic carbon; Pollution; Pollution levels; Research centers; Salt lakes; Sediment samplers; Sediments; Shellfish; Shellfish farming; Studies; Sulfate reduction; Sulfates; Sulfides
• ISSN: 0919-9268; 1444-2906
• DOI: 10.1007/s12562-011-0439-3

The abundance of sulphate-reducing bacteria (SRB) was investigated in the coastal marine sediments along the coast of Japan and South Korea. Sediment samples were collected from fish and shellfish farms between 2006 and 2008. As non-fish farming reference sites, sediments were also collected from highly eutrophic bays, a highly sulphidogenic saline lake, and the deep sea. A quantitative real-time PCR analysis that targeted the gene coding for a portion of the α-subunit of dissimilatory sulphite reductase ( dsrA ) was performed to assess the abundance of the SRB in the sediments. Between 2.8 × 10 7 and 2.5 × 10 9  copies of the dsrA gene per gram dry sediment were detected. There was no relationship between dsrA gene copy number and total bacterial count in the sediments, whereas organic matter contents (particulate organic carbon and nitrogen, ignition loss and chemical oxygen demand) and acid-volatile sulphide contents were significantly correlated with the dsrA copy number. The data presented demonstrate that organic enrichment of sediment may influence the abundance of SRB communities in coastal marine sediments and that the cell density of SRB may be used as a biological indicator for assessing pollution levels in sediments of marine fish farms.