Toxigenic Alternaria species from Argentinean blueberries

• Published in: International journal of food microbiology 2012-03, Vol.154 (3), p.187-191
• Main Authors: Greco, M., Patriarca, A., Terminiello, L., Fernández Pinto, V., Pose, G.
• Format: Article
• Language: English
• Subjects: Alternaria; Alternaria - isolation & purification; Alternaria - pathogenicity; Alternaria alternata; Alternaria tenuissima; Alternariol; Alternariol methylether; Argentina; Blueberries; Blueberry Plants - microbiology; byproducts; crops; Food Storage; host specificity; mycotoxins; Mycotoxins - biosynthesis; Oryza sativa; pathogenicity; Penicillium; Phoma; raw fruit; rice; risk; sporulation; Tenuazonic acid; Vaccinium
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2012.01.004

Blueberries are traditionally consumed in North America, some European countries and Japan. In Argentina, the blueberry crop is profitable because production starts in November, when the northern hemisphere lacks fresh fruit. Fungal contaminants can grow and produce mycotoxins in fresh fruit. The aims of this work were to identify the main genera of the mycobiota of blueberries grown in Argentina and to determine the toxicogenic potential, pathogenicity and host specificity of the species isolated. The genus Alternaria was the main component of the blueberry mycobiota (95%); minor proportions of Phoma spp. (4%) and Penicillium spp. (1%) were also isolated. According to their sporulation patterns, 127 Alternaria isolates belonged to the Alternaria tenuissima species-group, 5 to the Alternaria alternata species-group and 2 to the Alternaria arborescens species-group. The last mentioned species-group was not isolated at 5°C. Of the 134 isolates, 61% were toxicogenic in autoclaved rice; 97% of these produced alternariol (AOH) in a range from 0.14 to 119.18mg/kg, 95% produced alternariol methylether (AME) in a range from 1.23 to 901.74mg/kg and 65% produced tenuazonic acid (TA) in a range from 0.13 to 2778mg/kg. Fifty two isolates co-produced the three mycotoxins. According to the size of the lesion that they caused on blueberries, the isolates were classified as slightly pathogenic, moderately pathogenic and very pathogenic. No significant differences in pathogenicity were found on different blueberry varieties. In this work, high incidence and toxicogenic potential of the Alternaria isolates from blueberries were demonstrated. Thus, more studies should be done to evaluate the health risk posed by the presence of the Alternaria toxins in blueberries and in the manufactured by-products. ► The genus Alternaria was the main component of the blueberry mycobiota. ► A. tenuissima species-group was the most frequently isolated. ► Tenuazonic acid, alternariol and alternariol methylether were investigated. ► Toxicogenic potential of Alternaria from blueberries was demonstrated. ► Pathogenicity toward blueberries ranged from moderate to very pathogenic.