[NSI +] determinant has a pleiotropic phenotypic manifestation that is modulated by SUP35, SUP45, and VTS1 genes

We recently discovered the novel non-chromosomal determinant in Saccharomyces cerevisiae [NSI +] (nonsense suppression inducer), which causes omnipotent nonsense suppression in strains where the Sup35 N-terminal domain is deleted. [NSI +] possesses yeast prion features and does not correspond to pre...

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Bibliographic Details
Published in:Current genetics 2012-02, Vol.58 (1), p.35-47
Main Authors: Nizhnikov, Anton A, Magomedova, Zalina M, Rubel, Alexandr A, Kondrashkina, Alexandra M, Inge-Vechtomov, Sergey G, Galkin, Alexey P
Format: Article
Language:English
Subjects:
Base Sequence
Biochemistry
Biomedical and Life Sciences
Cell Biology
Codon, Nonsense
Gene Dosage
Gene Expression Regulation, Fungal
gene overexpression
genes
Genome, Fungal
Life Sciences
Microbial Genetics and Genomics
Microbiology
Molecular Sequence Data
Nonsense mutation
Peptide Termination Factors - genetics
Phenotype
Plant Sciences
Prion protein
prions
Prions - genetics
Proteomics
Research Article
RNA-binding protein
RNA-Binding Proteins - genetics
Saccharomyces cerevisiae
Saccharomyces cerevisiae - physiology
Saccharomyces cerevisiae Proteins - genetics
Suppression, Genetic
Translation
translation (genetics)
Translation termination
vegetative growth
Yeasts
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Summary:We recently discovered the novel non-chromosomal determinant in Saccharomyces cerevisiae [NSI +] (nonsense suppression inducer), which causes omnipotent nonsense suppression in strains where the Sup35 N-terminal domain is deleted. [NSI +] possesses yeast prion features and does not correspond to previously identified yeast prion determinants. Here, we show that [NSI + ] enhances nonsense codon read-through and inhibits vegetative growth in S. cerevisiae. Using a large-scale overexpression screen to identify genes that impact the phenotypic effects of [NSI +], we found that the SUP35 and SUP45 genes encoding the translation termination factors eRF3 and eRF1, respectively, modulate nonsense suppression in [NSI +] strains. The VTS1 gene encodes an NQ-enriched RNA-binding protein that enhances nonsense suppression in [NSI +] and [nsi −] strains. We demonstrate that VTS1 overexpression, like [NSI +] induction, causes translational read-through and growth defects in S. cerevisiae.
ISSN:0172-8083
1432-0983
DOI:10.1007/s00294-011-0363-1