Laminin active peptide/agarose matrices as multifunctional biomaterials for tissue engineering

Abstract Cell adhesive peptides derived from extracellular matrix components are potential candidates to afford bio-adhesiveness to cell culture scaffolds for tissue engineering. Previously, we covalently conjugated bioactive laminin peptides to polysaccharides, such as chitosan and alginate, and de...

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Bibliographic Details
Published in:Biomaterials 2012-06, Vol.33 (16), p.4118-4125
Main Authors: Yamada, Yuji, Hozumi, Kentaro, Aso, Akihiro, Hotta, Atsushi, Toma, Kazunori, Katagiri, Fumihiko, Kikkawa, Yamato, Nomizu, Motoyoshi
Format: Article
Language:English
Subjects:
Advanced Basic Science
Amino Acid Sequence
Animals
Biocompatible Materials
Cell Adhesion
Cell culture
Cells, Cultured
Dentistry
ECM (extracellular matrix)
Edetic Acid
Heparin
Humans
Laminin
Laminin - chemistry
Laminin - metabolism
Mice
Molecular Sequence Data
Neurites
PC12 Cells
Peptide
Peptides - chemistry
Peptides - metabolism
Polysaccharide
Rats
Sepharose - metabolism
Tissue Engineering
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Summary:Abstract Cell adhesive peptides derived from extracellular matrix components are potential candidates to afford bio-adhesiveness to cell culture scaffolds for tissue engineering. Previously, we covalently conjugated bioactive laminin peptides to polysaccharides, such as chitosan and alginate, and demonstrated their advantages as biomaterials. Here, we prepared functional polysaccharide matrices by mixing laminin active peptides and agarose gel. Several laminin peptide/agarose matrices showed cell attachment activity. In particular, peptide AG73 (RKRLQVQLSIRT)/agarose matrices promoted strong cell attachment and the cell behavior depended on the stiffness of agarose matrices. Fibroblasts formed spheroid structures on the soft AG73/agarose matrices while the cells formed a monolayer with elongated morphologies on the stiff matrices. On the stiff AG73/agarose matrices, neuronal cells extended neuritic processes and endothelial cells formed capillary-like networks. In addition, salivary gland cells formed acini-like structures on the soft matrices. These results suggest that the peptide/agarose matrices are useful for both two- and three-dimensional cell culture systems as a multifunctional biomaterial for tissue engineering.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2012.02.044