Strontium Ranelate Prevents Bone Loss in a Rat Model of Localized Muscle Paralysis

• Published in: Annals of biomedical engineering 2012-03, Vol.40 (3), p.657-665
• Main Authors: Sheng, Zhi-Feng, Ma, Yu-Lin, Tong, Dejun, Fang, De-Yu, Liang, Qing-Chun, Liu, Li-Hong, Zhang, Jian, Liao, Er-Yuan
• Format: Article
• Language: English
• Subjects: Animals; Biochemistry; Biological and Medical Physics; Biomechanical Phenomena; Biomedical and Life Sciences; Biomedical Engineering; Biomedical Engineering and Bioengineering; Biomedicine; Biophysics; Body weight; Bone Density - drug effects; Bone Density Conservation Agents - administration & dosage; Botulinum Toxins - toxicity; Classical Mechanics; Clostridium botulinum; Disease Models, Animal; Elastic Modulus; Female; Injection; Organometallic Compounds - administration & dosage; Osteoporosis - diagnostic imaging; Osteoporosis - etiology; Osteoporosis - physiopathology; Osteoporosis - prevention & control; Paralysis - chemically induced; Paralysis - complications; Paralysis - drug therapy; Rats; Rats, Sprague-Dawley; Strontium; Thiophenes - administration & dosage; Toxins; X-Ray Microtomography
• ISSN: 0090-6964; 1573-9686
• DOI: 10.1007/s10439-011-0406-2

Twenty-one 3.5-month-old female Sprague–Dawley rats were randomly assigned to three groups: BTX group, in which each rat received a single intramuscular injection of 2 U of Clostridium botulinum toxin (BTX) in the quadriceps femoris muscle of the right hind limb; BTX + SR group, in which each rat received a BTX injection and a dose of strontium ranelate (dose level of 625 mg/kg/day); and the control group. All the rats were killed at 9 weeks post-treatment. It was showed that BTX-induced rats a rapid loss of body weight in the first 3 weeks, after which their body weight showed a slow increase similar to that observed in the control rats. The net body weight loss was mainly attributed to muscle atrophy. BTX caused remarkable bone degradation in either the trabecular bone or the cortical bone of the disuse femur. The deteriorations in the bone mass and bone microstructure were locally limited and could be prevented by strontium ranelate treatment. Biomechanical analysis showed that strontium ranelate treatment improved the mechanical performance of the tibia in BTX-treated rats. It was showed that a clinical-corresponding dose of strontium ranelate could prevent bone loss in long-term immobilized rats.