A new xylanase from Streptomyces megasporus DSM 41476 with high yield of xylobiose

A new xylanase gene, xynBM4 , was cloned from Streptomyces megasporus DSM 41476 and expressed in Pichia pastoris . The full-length gene consists of 1,443 bp and encodes 480 amino acids including a putative 49-residue signal peptide. The deduced amino acid sequence of xynBM4 shows the highest identit...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2012-02, Vol.28 (2), p.687-692
Main Authors: Shi, Pengjun, Qiu, Zhenhua, Bai, Yingguo, Yuan, Tiezheng, Huang, Huoqing, Pan, Xia, Yang, Peilong, Zhang, Wei, Yao, Bin
Format: Article
Language:English
Subjects:
Adaptability
Agronomy
Amino Acid Sequence
Amino acids
Analysis
Applied Microbiology
Bacteria
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cloning
Disaccharides - biosynthesis
Endo-1,4-beta Xylanases - chemistry
Endo-1,4-beta Xylanases - genetics
Endo-1,4-beta Xylanases - metabolism
Environmental Engineering/Biotechnology
Enzymes
Experiments
Food
Genetic recombination
Hydrogen-Ion Concentration
Life Sciences
Microbiology
Molecular Sequence Data
Molecular weight
Original Paper
Peptides
Pichia pastoris
Streptomyces
Streptomyces - enzymology
Streptomyces - metabolism
Streptomyces halstedii
Studies
Substrate Specificity
Triticum aestivum
Yeast
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Summary:A new xylanase gene, xynBM4 , was cloned from Streptomyces megasporus DSM 41476 and expressed in Pichia pastoris . The full-length gene consists of 1,443 bp and encodes 480 amino acids including a putative 49-residue signal peptide. The deduced amino acid sequence of xynBM4 shows the highest identity of 66.3% to the xylanase Xys1L from Streptomyces halstedii JM8. The purified recombinant XYNBM4 had a high specific activity of 350.7 U mg -1 towards soluble wheat arabinoxylan, exhibited optimal activity at pH 6.0 and 57°C, showed broad pH adaptability (>75% of the maximum activity at pH 2.5–9.0), was resistant to neutral proteases and most chemicals, and produced simple products. The hydrolysis products of birchwood xylan and corncob xylan were predominantly xylobiose (76.9 and 90.8%, respectively) and no xylose. These characteristics suggest that XYNBM4 has potential in various applications, especially in the food industry.
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-011-0863-7