YAP1 over-expression in Saccharomyces cerevisiae enhances glutathione accumulation at its biosynthesis and substrate availability levels

Microbiological production of glutathione using genetically engineered yeast strains has a potential to satisfy the increasing industrial demand of this tripeptide. In the present work accumulation of glutathione in response to YAP1 over‐expression in Saccharomyces cerevisiae was studied. The over‐e...

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Bibliographic Details
Published in:Biotechnology journal 2012-04, Vol.7 (4), p.566-568
Main Authors: Orumets, Kerti, Kevvai, Kaspar, Nisamedtinov, Ildar, Tamm, Tiina, Paalme, Toomas
Format: Article
Language:English
Subjects:
Gene Expression Regulation, Fungal
Glutathione
Glutathione - metabolism
Metabolic engineering
S. cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Transcription Factors - genetics
Transcription Factors - metabolism
YAP1 over-expression
Yeast
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Summary:Microbiological production of glutathione using genetically engineered yeast strains has a potential to satisfy the increasing industrial demand of this tripeptide. In the present work accumulation of glutathione in response to YAP1 over‐expression in Saccharomyces cerevisiae was studied. The over‐expression resulted in intracellular glutathione level over two times higher than in the parent strain. Transcript analyses revealed that, in addition to the genes encoding enzymes in the glutathione biosynthesis pathway (GSH1 and GSH2), the expression levels of the genes in the cysteine biosynthesis pathway (CYS3 and CYS4) were also significantly higher in the YAP1 over‐expressed strain. This suggests that YAP1 over‐expression affects glutathione accumulation at both its biosynthesis and substrate availability levels. Microbiological production of glutathione using genetically engineered yeast strains has a potential to satisfy the increasing industrial demand of this tripeptide. In this work, the authors study the accumulation of glutathione in response to YAP1 over‐expression in Saccharomyces cerevisiae. The over‐expression of this single gene increases the intracellular pools of cysteine as well as the two enzymatic reactions leading to intracellular GSH accumulation.
ISSN:1860-6768
1860-7314
DOI:10.1002/biot.201100363