Discovery of DNA operators for TetR and MarR family transcription factors from Burkholderia xenovorans

Determining transcription factor (TF) recognition motifs or operator sites is central to understanding gene regulation, yet few operators have been characterized. In this study, we used a protein-binding microarray (PBM) to discover the DNA recognition sites and putative regulons for three TetR and...

Full description

Saved in:
Bibliographic Details
Published in:Microbiology (Society for General Microbiology) 2012-02, Vol.158 (Pt 2), p.571-582
Main Authors: TUHIN SUBHRA MAITY, CLOSE, Devin W, VALDEZ, Yolanda E, NOWAK-LOVATO, Kristy, MARTI-ARBONA, Ricardo, NGUYEN, Tinh T, UNKEFER, Pat J, HONG-GELLER, Elizabeth, BRADBURY, Andrew R. M, DUNBAR, John
Format: Article
Language:English
Subjects:
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteriology
Base Sequence
Binding Sites
Biological and medical sciences
Burkholderia
Burkholderia - chemistry
Burkholderia - classification
Burkholderia - genetics
Burkholderia - metabolism
Fundamental and applied biological sciences. Psychology
Microbiology
Miscellaneous
Molecular Sequence Data
Multigene Family
Operator Regions, Genetic
Phylogeny
Protein Binding
Transcription Factors - chemistry
Transcription Factors - genetics
Transcription Factors - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Determining transcription factor (TF) recognition motifs or operator sites is central to understanding gene regulation, yet few operators have been characterized. In this study, we used a protein-binding microarray (PBM) to discover the DNA recognition sites and putative regulons for three TetR and one MarR family TFs derived from Burkholderia xenovorans, which are common to the genus Burkholderia. We also describe the development and application of a more streamlined version of the PBM technology that significantly reduced the experimental time. Despite the genus containing many pathogenically important species, only a handful of TF operator sites have been experimentally characterized for Burkholderia to date. Our study provides a significant addition to this knowledge base and illustrates some general challenges of discovering operators on a large scale for prokaryotes.
ISSN:1350-0872
1465-2080
DOI:10.1099/mic.0.055129-0