Differential reactivity of serum immunoglobulins from Brazilian wild mammals to staphylococcal A and streptococcal G proteins

Human pathogens have evolved to infect vertebrate hosts other than human beings without causing symptoms of the disease, thus permitting them to complete their life cycle and to develop into infectious forms. The identification and management of infected animals are alternatives to control dissemina...

Full description

Saved in:
Bibliographic Details
Published in:Journal of veterinary diagnostic investigation 2012, Vol.24 (1), p.148-152
Main Authors: Pelli, Afonso, Castellano, Lucio R, Cardoso, Marcos R.S, Vasconcelos, Luis A.S, Domingues, Marcos A, Ferreira, Maria B, Rodrigues, Virmondes
Format: Article
Language:English
Subjects:
Animals
Animals, Wild - immunology
Animals, Wild - microbiology
Artiodactyla
Artiodactyla - immunology
Artiodactyla - microbiology
bacterial proteins
Bacterial Proteins - immunology
Brazil
Carnivora
Carnivora - immunology
Carnivora - microbiology
cats
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - veterinary
G-proteins
hosts
humans
immunoblotting
Immunoblotting - veterinary
immunoglobulins
Immunoglobulins - immunology
pathogens
protein binding
Rodentia
Rodentia - immunology
Rodentia - microbiology
serological surveys
Staphylococcus - immunology
Streptococcus
Streptococcus - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Human pathogens have evolved to infect vertebrate hosts other than human beings without causing symptoms of the disease, thus permitting them to complete their life cycle and to develop into infectious forms. The identification and management of infected animals are alternatives to control dissemination of the disease and to prevent human illness. In the current study, the potential use of staphylococcal A or streptococcal G proteins was evaluated with enzyme-linked immunosorbent assays (ELISAs) for seroepidemiological studies. Sera were collected from animals that were representative of 23 different Brazilian wild mammals. A high protein A binding rate was observed in all animals, except for the orders Didelphimorphia, Artiodactyla, and Rodentia, in which affinity was medium or low. Affinity for streptococcal G protein was higher in animals of the order Artiodactyla, whereas no streptococcal G protein binding was observed in samples obtained from felines (order Carnivora). Bacterial protein binding to mammalian immunoglobulins was confirmed by immunoblotting. The results suggest that secondary detection systems should be better investigated in ELISA protocols before their implementation in seroepidemiological studies involving wild mammals.
ISSN:1040-6387
1943-4936
DOI:10.1177/1040638711434322