A nested sequence-specific primer-polymerase chain reaction for the detection of HLA-B15:02

In this study, we reported a new technique in detecting HLA‐B*15:02 by using a nested sequence‐specific primer‐polymerase chain reaction (SSP‐PCR) that can be used on genomic DNA and whole blood for carbamazepine hypersensitivity prediction. We tested a total of 200 blind samples with known human le...

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Bibliographic Details
Published in:Tissue antigens 2012-04, Vol.79 (4), p.295-301
Main Authors: Virakul, S., Kupatawintu, P., Nakkuntod, J., Kangwanshiratada, O., Vilaivan, T., Hirankarn, N.
Format: Article
Language:English
Subjects:
Base Sequence
Blood
Carbamazepine
carbamazepine hypersensitivity
DNA Primers
genomics
Histocompatibility antigen HLA
Histocompatibility Testing - methods
HLA-B Antigens - chemistry
HLA-B Antigens - genetics
HLA-B15:02
Humans
Hypersensitivity
Molecular Sequence Data
nested sequence-specific primer-polymerase chain reaction
Nucleotide sequence
Oligonucleotides
Polymerase Chain Reaction
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Summary:In this study, we reported a new technique in detecting HLA‐B*15:02 by using a nested sequence‐specific primer‐polymerase chain reaction (SSP‐PCR) that can be used on genomic DNA and whole blood for carbamazepine hypersensitivity prediction. We tested a total of 200 blind samples with known human leukocyte antigen (HLA)‐B allelic types (44 positive for HLA‐B*15:02 and 156 negative for HLA‐B*15:02) with this new nested SSP‐PCR technique and compared its efficacy to that of commercial sequence‐specific oligonucleotide probe‐polymerase chain reaction (SSOP‐PCR). Using starting materials from DNA and whole blood, we were able to detect HLA‐B*15:02 in 44 of our samples correctly. The test is very sensitive and is highly reproducible.
ISSN:0001-2815
1399-0039
DOI:10.1111/j.1399-0039.2012.01836.x