Inter-simple-sequence-repeat (ISSR) polymorphisms are useful for finding markers associated with disease resistance gene clusters

• Published in: Theoretical and applied genetics 1998-09, Vol.97 (4), p.515-519
• Main Authors: Ratnaparkhe, M.B, Tekeoglu, M, Muehlbauer, F.J
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Chickpea; Chromosome mapping; Cicer; Cicer arietinum; cicer reticulatum; Classical genetics, quantitative genetics, hybrids; disease resistance; Fundamental and applied biological sciences. Psychology; fungal diseases of plants; Fusarium; Genes; Genetic aspects; Genetic markers; Genetics; Genetics of eukaryotes. Biological and molecular evolution; Identification and classification; inheritance (genetics); linkage groups; microsatellite repeats; Microsatellites (Genetics); pathotypes; Plant genetics; Plant immunology; Pteridophyta, spermatophyta; repetitive sequences; segregation; Vegetals; wild relatives; wilts
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220050925

We describe a simple and new approach, based on inter-simple sequence repeats (ISSRs), for finding markers linked to clusters of disease resistance genes. In this approach, simple sequence repeats (SSR) are used directly in PCR reactions, and markers found to be linked to disease resistance genes provide important information for the selection of other sequences which can be used with PCR to find other linked markers. Based on an ISSR marker linked to a gene of interest, many new markers can be identified in the same region. We previously demonstrated that ISSR markers are useful in gene tagging and identified a marker, UBC-855(500), linked to the gene for resistance to fusarium wilt race 4 in chickpea. This ISSR marker provided the information used in the present study for selecting other primers which amplified a region linked to the gene for resistance to fusarium wilt race 4. The primers were based on homology with the (AC)n sequence and were used for PCR amplifications. Changes in the sequence were at the anchor region of the primers. The repeat (AC)8T amplified a marker, UBC-825(1200), which was located 5.0 cM from the gene for resistance to fusarium wilt race 4 and was closer than other markers. These results indicated that ISSR markers can provide important information for the design of other primers and that by making changes at the 3' and 5' anchors close linkage to the desired gene can be found. The approach allows rapid scanning of the targeted region and may provide important information for genome analysis of plant species.