Genetic analysis of inbreeding depression in plus tree 850.55 of Pinus radiata D. Don. II. Genetics of viability genes

• Published in: Theoretical and applied genetics 1999-07, Vol.99 (1/2), p.140-146
• Main Authors: Kuang, H, Richardson, T.E, Carson, S.D, Bongarten, B.C
• Format: Article
• Language: English
• Subjects: algorithms; alleles; Biological and medical sciences; Chi-square test; chromosome mapping; Classical genetics, quantitative genetics, hybrids; dominance; expectation conditional maximization algorithm; Fundamental and applied biological sciences. Psychology; gene location; Genes; genetic markers; genetic recombination; Genetics of eukaryotes. Biological and molecular evolution; Genomes; Genotype & phenotype; inbreeding depression; incomplete dominance; lethal genes; linkage (genetics); Maximum likelihood method; microsatellite repeats; overdominance; Pinus radiata; plus trees; Pteridophyta, spermatophyta; random amplified polymorphic DNA technique; seed set; Seeds; segregation; segregation distortion; Trees; Vegetals; viability
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220051218

Most coniferous species exhibit severe inbreeding depression. Selfed individuals usually have decreased viability, reduced vigour and morphological defects. The number of filled seeds after selfing Pinus radiata plus tree 850.55 was 48% that of the outcrossing, and 26.1% of the selfed seedlings died at an early stage. The segregation of 172 markers (covering 56% of the genome) in selfed progenies of radiata pine plus tree 850.55 was studied. Based on the segregation ratio of the markers, genes associated with inbreeding depression on viability were identified (P < 0.05). Using the Expectation/Conditional Maximization (ECM) algorithm, we estimated the location, degree of dominance and selection coefficient of viability genes. Nine viability genes were discovered. Seven of them appeared to be dominant and one partially dominant (degree of dominance = 0.4). The other gene was overdominant or pseudo-overdominant, with selection coefficients for the two homozygotes of 0.4 and 0.42, respectively. Of the genes showing dominance or partial dominance, seven were sub-lethal with selection coefficients ranging from 0.55 to 0.79; one gene (SDPr), which was responsible for seedling death within the first month following germination, was lethal.